{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T02:40:46Z","timestamp":1699843246627},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9841,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1979,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As a means of investigating the immunoglobulin (Ig) deficiency in multiple myeloma, the effects of pokeweed mitogen (PWM) on morphologic transformation and concomitant immunoglobulin synthesis and secretion kinetics were studied in peripheral blood lymphocytes (PBL) of normals and patients with myeloma. Lymphocyte Ig production kinetics, both in the unstimulated state and after five days of culture with PWM, were evaluated by <jats:sup>3<\/jats:sup>H\u2010leucine incorporation. B\u2010lymphocytes were found to be decreased by EC3 rosetting in the myeloma patients studied. In addition their lymphocytes had a mean of 30% transformation compared to 53% for normals (P &lt; 0.005). After five days of mitogen stimulation in culture, myeloma PBL failed to show the rise in intracellular Ig production seen in normal PBL under similar conditions. Thus, myeloma patients were found to have decreased numbers of EC3 rosetting B\u2010cells, decreased morphologic transformation rate in response to mitogenic stimulation, and failure to increase intracellular Ig after mitogenic stimulation. Possible etiologic mechanisms for the decreased Ig production are discussed.<\/jats:p>","DOI":"10.1002\/ajh.2830060408","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T05:22:47Z","timestamp":1172035367000},"page":"361-371","source":"Crossref","is-referenced-by-count":1,"title":["The kinetics of immunoglobulin synthesis by pokeweed mitogen stimulated peripheral blood lymphocytes in multiple myeloma"],"prefix":"10.1002","volume":"6","author":[{"given":"Edward R.","family":"George","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey J.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(63)90140-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1182\/blood.V33.2.300.300","article-title":"Abnormal nucleic acid metabolism of lymphocytes in plasma cell myeloma and macroglobulinemia","volume":"33","author":"Salmon SE","year":"1969","journal-title":"Blood"},{"key":"e_1_2_1_4_2","unstructured":"WarnerNC MetcalfD: \u201cMultiple Myeloma and Related Immunoglobulin\u2010Producing Neoplasms.\u201d UICC Technical Report Series 13:63 1974."},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1182\/blood.V30.3.265.265","article-title":"Myeloma proteins, Bence Jones proteins and normal immmunoglobulins in multiple myeloma","volume":"30","author":"Caggiano V","year":"1967","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108736"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb00995.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105098"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.4049\/jimmunol.108.4.1039","article-title":"The effect of plasmacytomas on the immune response of mice","volume":"108","author":"Zolla S","year":"1972","journal-title":"J Immunol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.4049\/jimmunol.112.6.2068","article-title":"Cellular basis of immunodepression in mice with plasmacytomas","volume":"112","author":"Zolla S","year":"1974","journal-title":"J Immunol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"702","DOI":"10.4049\/jimmunol.118.2.702","article-title":"Suppression of the humoral immune response by plasmacytomas: Mediation by adherent mononuclear cells","volume":"118","author":"Kolb JP","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.123.4.394"},{"key":"e_1_2_1_13_2","first-page":"1","article-title":"Metabolism of normal 6.6S \u03c3\u2010globulin in normal subjects and patients with macroglobulinemia and multiple myeloma","volume":"62","author":"Solomon A","year":"1963","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_14_2","first-page":"1","article-title":"Metabolism of immunoglobulins","volume":"13","author":"Waldmann TA","year":"1969","journal-title":"Prog Allergy"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-83-5-625"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-78-6-837"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-83-1-42"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1974.tb14453.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197510302931801"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.4049\/jimmunol.118.4.1385","article-title":"Hypogammaglobulinemia in experimental myeloma: The role of suppressor factors from mononuclear phagocytes","volume":"118","author":"Krakauer RS","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1977.tb00371.x"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.4049\/jimmunol.118.5.1864","article-title":"Multiple myeloma: An immunologic profile. 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