{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:50Z","timestamp":1762067150034},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9780,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1979,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In cell free systems Adriamycin induces oxygen radicals. We have shown previously that Adriamycin generates peroxide in human erythrocytes. BCNU, by inhibiting glutathione reductase, interferes with the major erythrocyte pathway to degrade peroxide. In this investigation we looked at interactions of these drugs with normal and abnormal erythrocytes.<\/jats:p><jats:p>In G6PD\u2010deficient erythrocytes Adriamycin posed a significant oxidant stress as demonstrated by hexose monophosphate shunt (HMPS) activity, hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>) production, and glutathione depletion. At similar molar concentrations Adriamycin was a stronger oxidant than acetylphenylhydrazine.<\/jats:p><jats:p>BCNU\u2010treated normal erythrocytes showed an enhanced susceptibility to oxidant stress as demonstrated by a lack of HMPS response to H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and glutathione depletion during incubations with Adriamycin. The HMPS shunt of BCNU treated RBC was intact as shown by their nearly normal response to methylene blue stimulation. These BCNU studies also demonstrated the inability of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> to react directly with NADPH.<\/jats:p><jats:p>In conclusion Adriamycin poses a potent oxident stress to G6PD\u2010deficient erythrocytes. BCNU promotes enhanced susceptibility of normal RBC to oxidant stress and BCNU can act as a probe to define drug interactions with the HMPS. These studies add to a growing body of evidence postulating the importance of oxygen radicals in the therapeutic and\/or effects of Adriamycin.<\/jats:p>","DOI":"10.1002\/ajh.2830070202","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T05:59:14Z","timestamp":1172037554000},"page":"97-106","source":"Crossref","is-referenced-by-count":25,"title":["The effect of BCNU and adriamycin on normal and G6PD deficient erythrocytes"],"prefix":"10.1002","volume":"7","author":[{"suffix":"Jr.","given":"Arthur L.","family":"Sagone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Mark","family":"Burton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00906a038"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00895a006"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-80-2-249"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1182\/blood.V52.5.878.878","article-title":"Adriamycin and daunomycin generate reactive oxygen compounds in erythrocytes","volume":"52","author":"Henderson CA","year":"1978","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(77)90543-5"},{"key":"e_1_2_1_7_2","first-page":"1080","article-title":"Severe generalized glutathione reductase deficiency after antitumor chemotherapy with BCNU","volume":"89","author":"Frischer H","year":"1977","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108531"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(72)90306-6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1016\/S0021-9258(18)62842-X","article-title":"A mechanism for the production of ethylene from methional. The generation of hydroxyl radical by xanthine oxidase","volume":"245","author":"Beauchamp C","year":"1970","journal-title":"J Biol Chem"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1978.tb07039.x"},{"key":"e_1_2_1_12_2","first-page":"882","article-title":"Improved method for the determination of blood glutathione","volume":"61","author":"Beutler E","year":"1963","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_13_2","first-page":"832","article-title":"The mechanism of oxidative hemolysis produced by phenylhydrazine","volume":"13","author":"Goldberg B","year":"1977","journal-title":"Mol Pharm"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","unstructured":"CarsonPE FroscherH ParkhurstGW HohlR AhmedT ScanlanS ChuL McKannaR:Drug stimulation of glucose oxidation in human erythrocytes: effect of glutathione reductase deficiency after BCNU. \u201cProc 7th Int Cong Pharm \u201d p396 1978.","DOI":"10.1016\/B978-0-08-023768-8.51037-9"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830070202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830070202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:03:20Z","timestamp":1699844600000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830070202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,10]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,10]]}},"alternative-id":["10.1002\/ajh.2830070202"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830070202","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,10]]}}}