{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:32:09Z","timestamp":1770215529577,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9780,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1979,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bleeding times of mink with the Chediak\u2010Higashi (CH) syndrome was markedly prolonged. Platelet counts were normal but there was an impaired platelet aggregation response to collagen. The metabolic adenine nucleotide pool of platelets from normal and CH mink was labeled with <jats:sup>14<\/jats:sup>C\u2010adenine and the platelets were gel\u2010filtered. Gel\u2010filtered platelets (GFP) from CH mink contained only 37.9% of the adenosine triphosphate (ATP) and 9.6% of the adenosine diphosphate (ADP) found in normal platelets and the ATP\/ADP ratio was similar to the <jats:sup>14<\/jats:sup>C\u2010ATP\/<jats:sup>14<\/jats:sup>C\u2010ADP ratio. Platelet content of Ca<jats:sup>2+<\/jats:sup>, Mg<jats:sup>2+<\/jats:sup>, and in particular 5\u2010hydroxytryptamine was decreased. When GFP were incubated with thrombin to induce maximal secretion, only negligible amounts of ATP and ADP were released. The specific activity of the extracellular nucleotides approximated that within the platelet. These findings suggest that the stored nucleotide pool in CH platelets is virtually absent and that the abnormalities in platelet function may be due, in part, to the essential absence of secretable ADP and serotonin. The release of Ca<jats:sup>2+<\/jats:sup> and Mg<jats:sup>2+<\/jats:sup> by CH platelets was 56% and 27.8% of normal, respectively.<\/jats:p>","DOI":"10.1002\/ajh.2830070206","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T05:59:14Z","timestamp":1172037554000},"page":"137-146","source":"Crossref","is-referenced-by-count":19,"title":["Characterization of platelets from normal mink and mink with the Chediak\u2010Higashi syndrome"],"prefix":"10.1002","volume":"7","author":[{"given":"K. M.","family":"Meyers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Holmsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. L.","family":"Seachord","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Hopkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Gorham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-197207000-00001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/49.3.505"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1093\/oxfordjournals.jhered.a107620","article-title":"Giant granules in leukocytes of the beige mouse","volume":"8","author":"Lutzner MA","year":"1967","journal-title":"J Hered"},{"key":"e_1_2_1_5_2","first-page":"861","article-title":"An inherited condition of enlarged leukocytic and melanin granule in cats: Probable homolog with the Chediak\u2010Higashi syndrome","volume":"34","author":"Kramer JW","year":"1975","journal-title":"Fed Proc"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1182\/blood.V22.4.477.477","article-title":"Studies of abnormal leukocyte bodies in mink","volume":"22","author":"Leader RW","year":"1968","journal-title":"Blood"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1182\/blood.V48.2.175.175","article-title":"Decreased nucleotide and serotonin storage associated with defective function in Chediak\u2010Higashi syndrome in cattle and human platelets","volume":"48","author":"Bell TG","year":"1976","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1182\/blood.V47.6.941.941","article-title":"Platelet function in the Chediak\u2010Higashi syndrome","volume":"47","author":"Buchanan GR","year":"1976","journal-title":"Blood"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb00618.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V20.3.330.330"},{"key":"e_1_2_1_11_2","first-page":"337A","article-title":"5\u2010Hydroxtryptamine metabolism, alpha granule release, and life span of Chediak\u2010Higashi syndrome platelets","volume":"25","author":"Corash L","year":"1977","journal-title":"Clin Res"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1182\/blood.V48.4.517.517","article-title":"A platelet abnormality in the Chediak\u2010Higashi syndrome of man","volume":"48","author":"Costa JL","year":"1976","journal-title":"Blood"},{"key":"e_1_2_1_13_2","unstructured":"MeyersKM HolmsenH SeachordCL HopkinsG BorchardR PadgettGA:Storage pool deficiency in platelets from Chediak\u2010Higashi cattle. Am J Physiol(In press)."},{"key":"e_1_2_1_14_2","first-page":"375","article-title":"A platelet serotonin anomaly in the Chediak\u2010Higashi syndrome","volume":"7","author":"Meyers KM","year":"1974","journal-title":"Res Commun Chem Pathol Pharmacol"},{"key":"e_1_2_1_15_2","first-page":"197","article-title":"Ultrastructural and morphometric studies of platelets from cattle with the Chediak\u2010Higashi syndrome","volume":"35","author":"Prieur DJ","year":"1976","journal-title":"Lab Invest"},{"key":"e_1_2_1_16_2","first-page":"108","volume-title":"The Diagnosis of Bleeding Disorders","author":"Owen CA","year":"1975"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1182\/blood.V34.2.204.204","article-title":"The standardized normal Ivy bleeding time and its prolongation by aspirin","volume":"34","author":"Milke CH","year":"1969","journal-title":"Blood"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197207272870401"},{"key":"e_1_2_1_19_2","first-page":"811","article-title":"Studies of gel\u2010filtered human platelets: Isolation and characterization in a medium containing no added Ca2+, Mg2+, or K2+","volume":"85","author":"Lages B","year":"1975","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(73)90068-3"},{"key":"e_1_2_1_21_2","unstructured":"MeyersKM HolmsenH SeachordCL HopkinsG KatzJ:An assessment of serotonin and arachidonate oxidation by platelets from normal cattle and cattle with the Chediak\u2010Higashi syndrome. (Submitted for publication.)"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-151-39137"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-142-36951"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V46.1.131.131"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(73)90114-7"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/222991a0"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1152\/ajplegacy.1973.224.4.941","article-title":"Effects of external Ca2+ and Mg2+ on thrombin\u2010induced changes in Ca2+ and Mg2+ of pig platelets","volume":"224","author":"Kinlough\u2010Rathbone RL","year":"1973","journal-title":"Am J Physiol"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1182\/blood.V46.1.119.119","article-title":"Metal ion content of gel\u2010filtered platelets from patients with storage pool disease","volume":"45","author":"Lages B","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1968.tb08487.x"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107778"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.165.3896.913"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830070206","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830070206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:03:48Z","timestamp":1699844628000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830070206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,10]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,10]]}},"alternative-id":["10.1002\/ajh.2830070206"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830070206","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,10]]}}}