{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T21:51:46Z","timestamp":1757541106911},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9536,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1980,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sickle (Hb SS) red cells, preloaded with <jats:sup>45<\/jats:sup>Ca by reversal of hemolysis, exhibit an incomplete <jats:sup>45<\/jats:sup>Ca extrusion, retaining approximately four times more <jats:sup>45<\/jats:sup>Ca than normal cells. Studies indicated that neither the reduction in Hb SS cell Ca<jats:sup>2+<\/jats:sup>\u2010Mg<jats:sup>2+<\/jats:sup> ATPase activity (84% of normal) nor the activation of Ca<jats:sup>2+<\/jats:sup>\u2010Mg<jats:sup>2+<\/jats:sup> ATPase by calmodulin was sufficiently different from normal cells to attribute a major role to the calcium pump in <jats:sup>45<\/jats:sup>Ca retention. These results suggested that <jats:sup>45<\/jats:sup>Ca retention may reflect an alteration in the calcium\u2010binding properties of Hb SS cell membranes. Low\u2010affinity calcium\u2010 binding (freely dissociable) was similar in normal and Hb SS cell membranes. However, the total calcium bound with high\u2010affinity (tightly bound) was four\u2010to\u2010five times greater in Hb SS cell membranes than in normal membranes. These results are compatible with the hypothesis that Hb SS cell <jats:sup>45<\/jats:sup> Ca retention reflects an exchange of a fraction of the total <jats:sup>45<\/jats:sup>Ca with a tightly bound calcium pool, larger in Hb SS cell membranes than in normal membranes.<\/jats:p><jats:p>A comparable degree of red cell <jats:sup>45<\/jats:sup>Ca retention, which did not correlate with the reticulocyte population, was observed in other chronic anemic states. These findings suggest that the increased high\u2010affinity calcium binding by the membrane may be a consequence of cellular changes induced by the anemic condition.<\/jats:p>","DOI":"10.1002\/ajh.2830080406","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T05:31:50Z","timestamp":1172035910000},"page":"377-387","source":"Crossref","is-referenced-by-count":11,"title":["Sickle red cell calcium metabolism: Studies on Ca<sup>2+<\/sup>\u2010Mg<sup>2+<\/sup>ATPase and ca\u2010binding properties of sickle red cell membranes"],"prefix":"10.1002","volume":"8","author":[{"given":"Irene","family":"Litosch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang 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