{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:58:00Z","timestamp":1756994280170},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9444,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1980,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Granulopoietic assessment was made in 14 patients with chronic idiopathic neutropenia (CIN) whose neutrophils were consistently less than 1.5 \u00d7 10<jats:sup>9<\/jats:sup>\/ liter and in whom there was absence of splenomegaly and recent drug ingestion. Granulopoiesis was studied using a combination of bone marrow culture in nutrient agar and granulocyte kinetics. Agar colony growth assessed bone marrow concentration of granulocyte progenitor cells (GPC) and the proportion of GPC in DNA synthesis by in vitro <jats:sup>3<\/jats:sup>HTdR suicide. Granulocyte kinetics with in vitro DF<jats:sup>32<\/jats:sup>P labelling of patient granulocytes measured granulocyte half\u2010life (T<jats:sup>1\/2<\/jats:sup>), turnover rate (GTR), and the circulating, marginated, and total blood granulocyte pools. The results indicated that either GPC concentration or the proportion of GPC in DNA synthesis was outside the normal range in all but one patient and decreased in ten out of 14 patients. CIN was also characterized by reduced total, circulating, and marginated blood granulocyte pools, reduced GTR, and normal granulocyte half\u2010life. The neutropenia appeared to be due to a variety of intra\u2010marrow causes, including either a reduction in the GPC compartment, a reduction in GPC proliferation, a maturation arrest, or a reduced amplication during granulopoiesis. Increased granulocyte utilization, intra\u2010vascular destruction or excessive margination could be excluded as possible causes of CIN in this series. Although GPC parameters tended to be reduced, suggesting a production defect, there were signs in nine patients that the bone marrow was attempting to compensate for the peripheral neutropenia. It is suggested that for a complete assessment of granulopoiesis in man, granulocyte kinetic studies need to be combined with quantitative studies of the bone marrow granulocyte progenitor compartment using the agar colony system.<\/jats:p>","DOI":"10.1002\/ajh.2830090207","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T06:48:32Z","timestamp":1172040512000},"page":"193-200","source":"Crossref","is-referenced-by-count":8,"title":["Chronic idiopathic neutropenia: Granulopoietic assessment by both marrow culture and granulocyte kinetics"],"prefix":"10.1002","volume":"9","author":[{"given":"K. A.","family":"Rickard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. D.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Yuen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kronenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"417","volume-title":"Clinical Haematology in Medical Practice","author":"de Gruchy GC","year":"1978"},{"key":"e_1_2_1_3_2","first-page":"413","volume-title":"Haematology \u2010 Tutorials in Postgraduate Medicine","author":"Goldman JM","year":"1975"},{"key":"e_1_2_1_4_2","first-page":"628","volume-title":"Hematology","author":"Finch SC","year":"1972"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(52)90079-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196811072791902"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1964.tb40718.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106657"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb05748.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1182\/blood.V14.4.303.303","article-title":"Leukokinetic studies. 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A method for labeling leukocytes with diisopropylfluorophosphate (DFP32)","volume":"14","author":"Athens JW","year":"1959","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104167"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104230"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104338"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1182\/blood.V36.1.98.98","article-title":"An improved method for performing neutrophil survival studies","volume":"36","author":"Deinard AS","year":"1970","journal-title":"Blood"},{"key":"e_1_2_1_15_2","volume-title":"Personal communication","author":"Vincent PC"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1966.28"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-5994.1973.tb03107.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V30.4.474.474"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1152\/jappl.1965.20.4.803","article-title":"Neutrophilic granulocyte kinetics in normal man","volume":"20","author":"Alexanian R","year":"1964","journal-title":"J Appl Physiol"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830090207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830090207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T22:54:56Z","timestamp":1699829696000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830090207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,9]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1980,9]]}},"alternative-id":["10.1002\/ajh.2830090207"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830090207","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,9]]}}}