{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:19:47Z","timestamp":1761153587185},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9202,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1981,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of short\u2010term cryopreservation on metabolic, functional, and survival characteristics of erythrocytes from patients with sickle cell disease was examined. Post\u2010thaw hemolysis of glycerolized sickle (SS) erythrocytes was greater (mean 12.9%) than in hemoglobin\u2010AA cells (mean 4.7%). Freeze preservation had no apparent effect on red cell morphology, percent irreversibly sickled cells, and fetal hemoglobin content. There were modest reductions in ATP and 2,3\u2010diphosphoglycerate in thawed, washed sickle erythrocytes (12.7% and 29.7%, respectively). However, the autologous survival of <jats:sup>51<\/jats:sup> Cr\u2010labelled SS red cells was not shortened by cryopreservation. The safety and efficacy of autotransfusion of cryopreserved red cells in alloimmunized sickle cell disease patients with anemic episodes unrelated to sickling need to be determined.<\/jats:p>","DOI":"10.1002\/ajh.2830100309","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T06:42:33Z","timestamp":1172040153000},"page":"297-304","source":"Crossref","is-referenced-by-count":15,"title":["Freeze preservation of sickle erythrocytes"],"prefix":"10.1002","volume":"10","author":[{"given":"Oswaldo","family":"Castro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn Phillips","family":"Hardy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William P.","family":"Winter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marne","family":"Hornblower","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold T.","family":"Meryman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830050204"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1980.20681057159.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1976.16677060246.x"},{"key":"e_1_2_1_5_2","first-page":"527","article-title":"The laboratory diagnosis of the haemoglobinopathies","volume":"3","author":"Efremov GD","year":"1974","journal-title":"Clin Haematol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.1972.tb00001.x"},{"key":"e_1_2_1_7_2","first-page":"413","article-title":"Studies on abnormal hemoglobins. 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Their demonstration in sickle cell anemia and other disorders by means of alkali denaturation","volume":"6","author":"Singer K","year":"1951","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1182\/blood.V38.3.378.378","article-title":"Recommended methods for radioisotope red cell survival studies","volume":"38","author":"The International Committee for Standardization in Hematology","year":"1971","journal-title":"Blood"},{"key":"e_1_2_1_9_2","first-page":"612","volume-title":"Nuclear Medicine","author":"Blahd WH","year":"1971"},{"issue":"1","key":"e_1_2_1_10_2","first-page":"66a","article-title":"Protein biosynthesis in sickle red blood cells following storage in glycerol at \u201370\u00b0C, (abstracted)","volume":"54","author":"Winter WP","year":"1979","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb00556.x"},{"key":"e_1_2_1_12_2","first-page":"5","volume-title":"Clinical Uses of Frozen\u2010Thawed Red Blood Cells","author":"Brewer GJ","year":"1976"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107199"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1182\/blood.V46.4.583.583","article-title":"Iron metabolism, sickle cell disease, and response to cyanate","volume":"46","author":"Peterson CM","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1970.tb01919.x"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830100309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830100309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:12:11Z","timestamp":1699834331000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830100309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,5]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1981,5]]}},"alternative-id":["10.1002\/ajh.2830100309"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830100309","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,5]]}}}