{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:34:33Z","timestamp":1718656473600},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9110,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1981,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Potassium efflux from deoxygenated, hemoglobin S\u2010containing red cells is often used as an \u201cobjective\u201d in vitro measure of red cell sickling, particularly during tests with antisickling agents. Since varying pH is known to affect both the extent of sickling and passive K<jats:sup>+<\/jats:sup>\u2010flux across the red cell membrane, in opposite directions, we measured the sickling\u2010related K<jats:sup>+<\/jats:sup>\u2010efflux in sickle cell anemia (SS) and sickle cell trait (AS) red cells as a function of extracellular and intracellular pH. The sickling\u2010related K<jats:sup>+<\/jats:sup>\u2010efflux was found to show the same direction of pH dependence as normal red cells, so that as the extracellular pH was reduced below 7.6, sickling and K<jats:sup>+<\/jats:sup>\u2010efflux were increasingly dissociated. A similar dissociation was observed between sickling and K<jats:sup>+<\/jats:sup>\u2010efflux when the intracellular pH was lowered by increasing red cell organic phosphate levels. The sickling\u2010related K<jats:sup>+<\/jats:sup>\u2010efflux from osmotically shrunken AS cells (whose sickling tendency resembles that of SS cells) was similar in magnitude and pH dependency to that of the SS cells. The findings suggest that measurement of K<jats:sup>+<\/jats:sup>\u2010efflux may be an accurate estimate of the extent of intracellular polymerization in sickled red cells, provided that both the intracellular and extracellular pH levels are carefully controlled, and the experimental conditions produce no independent effects on K<jats:sup>+<\/jats:sup> permeability.<\/jats:p>","DOI":"10.1002\/ajh.2830110104","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T06:38:55Z","timestamp":1172039935000},"page":"19-27","source":"Crossref","is-referenced-by-count":6,"title":["pH dependency of potassium efflux from sickled red cells"],"prefix":"10.1002","volume":"11","author":[{"suffix":"Jr.","given":"Eugene F.","family":"Roth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald L.","family":"Nagel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Bookchin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197207132870201"},{"key":"e_1_2_1_3_2","first-page":"169","volume-title":"Development of Therapeutic Agents for Sickle Cell Disease","author":"Natta CL","year":"1979"},{"key":"e_1_2_1_4_2","first-page":"179","volume-title":"Development of Therapeutic Agents for Sickle Cell Disease","author":"Lubin BH","year":"1979"},{"key":"e_1_2_1_5_2","first-page":"187","volume-title":"Development of Therapeutic Agents for Sickle Cell Disease","author":"Manning JM","year":"1979"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.39.1.31"},{"key":"e_1_2_1_7_2","first-page":"576","volume-title":"Oxygen Affinity of Hemoglobin and Red Cell Acid\u2010Base Status","author":"Deuticke B","year":"1972"},{"key":"e_1_2_1_8_2","first-page":"597","article-title":"Determinants of red cell sickling. Effects of varying pH and of increasing intracellular hemoglobin concentration by osmotic shrinkage","volume":"87","author":"Bookchin RM","year":"1976","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_9_2","first-page":"71","volume-title":"The Red Blood Cell","author":"Passow H","year":"1964"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(74)90233-8"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(80)90301-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1981.sp013628"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/246105a0"},{"key":"e_1_2_1_14_2","first-page":"1365","article-title":"Red cell calcium contents and trans membrane calcium movements in sickle cell anemia","volume":"89","author":"Palek J","year":"1977","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/284561a0"},{"key":"e_1_2_1_16_2","first-page":"217","volume-title":"Current Topics in Membranes and Transport","author":"Lew VL"},{"key":"e_1_2_1_17_2","first-page":"35","article-title":"Effect of propranalol on the Ca\u2010dependent K permeability of red cell membranes","volume":"300","author":"Simons TJB","year":"1980","journal-title":"J Physiol (Lond)"},{"key":"e_1_2_1_18_2","first-page":"47","volume-title":"Development of Therapeutic Agents for Sickle Cell Disease","author":"Bookchin RM","year":"1979"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-677202-9.50008-3"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830110104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830110104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T23:30:45Z","timestamp":1699831845000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830110104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,8]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1981,8]]}},"alternative-id":["10.1002\/ajh.2830110104"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830110104","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,8]]}}}