{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:26:20Z","timestamp":1791289580402,"version":"4.3.0"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":9018,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1981,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To assess the capacity of the human spleen to function as a hematopoietic organ, spleen mononuclear cells from patients undergoing splenectomy were cultured to assay CFU\u2010E, BFU\u2010E, and CFU\u2010C. Although the peripheral blood from cases of hereditary spherocytosis, Gaucher disease, Thalassemia major, and idiopathic thrombocytopenic purpura yielded 3\u201320 BFU\u2010E\/5 \u00d7 10<jats:sup>5<\/jats:sup> cells plated and 1\u20137 CFU\u2010C\/5 \u00d7 10<jats:sup>5<\/jats:sup>, suspensions of spleen cells failed to grow colonies. Histologically these spleens did not show extramedullary hematopoiesis. In contrast, peripheral blood from a patient with osteopetrosis (marble bone disease) yielded 130 BFU\u2010E\/5 \u00d7 10<jats:sup>5<\/jats:sup>, 40 CFU\u2010E\/5 \u00d7 10<jats:sup>5<\/jats:sup>, and 9 CFU\u2010C\/5 \u00d7 10<jats:sup>5<\/jats:sup>. The spleen had extensive extramedullary hematopoiesis on microscopy and grew 180 BFU\u2010E\/10<jats:sup>5<\/jats:sup>, 210 CFU\u2010E\/10<jats:sup>5<\/jats:sup>, and 25 CFU\u2010C\/10<jats:sup>5<\/jats:sup>. We conclude that spleens from patients without extramedullary hematopoiesis do not contain committed hematopoietic progenitors in spite of normal precursors in the blood. In osteopetrosis the spleen contained stem cells in a concentration comparable to bone marrow, and the embryonic role of the spleen in blood formation appeared to continue in postnatal life.<\/jats:p>","DOI":"10.1002\/ajh.2830110307","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T06:31:15Z","timestamp":1172039475000},"page":"271-275","source":"Crossref","is-referenced-by-count":35,"title":["Hematopoiesis in the human spleen"],"prefix":"10.1002","volume":"11","author":[{"given":"Melvin H.","family":"Freedman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Fred","family":"Saunders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"2","volume-title":"Hematologic Problems in the Newborn","author":"Oski FA","year":"1972"},{"key":"e_1_2_1_3_2","first-page":"28","volume-title":"Clinical Hematology","author":"Wintrobe MM","year":"1967"},{"key":"e_1_2_1_4_2","first-page":"2376","article-title":"Myeloid metaplasia in Hodgkin's disease","volume":"59","author":"Levitan R","year":"1959","journal-title":"NY State J Med"},{"key":"e_1_2_1_5_2","first-page":"154","article-title":"Extramedullary hemapoiesis. Myeloid metaplasia","volume":"12","author":"Rosenthal M","year":"1950","journal-title":"Bull N Engl Med Cen"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(65)80316-X"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040830218"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1182\/blood.V37.1.1.1","article-title":"Colony formation by normal and leukemic human marrow cells in culture: Effect of conditioned medium from human leukocytes","volume":"37","author":"Iscove NN","year":"1971","journal-title":"Blood"},{"issue":"4","key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1182\/blood.V47.4.531.531","article-title":"Granulopoiesis in severe congenital neutropenia","volume":"47","author":"Amato D","year":"1976","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1182\/blood.V49.6.855.855","article-title":"Human marrow cells capable of erythropoietic differentiation in vitro.: Definition of three erythroid colony responses","volume":"49","author":"Gregory CJ","year":"1977","journal-title":"Blood"},{"key":"e_1_2_1_11_2","first-page":"455","article-title":"A prenatal diagnosis of osteopetrosis","volume":"49","author":"Jenkinson EL","year":"1943","journal-title":"Am J Roentgenol"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830110307","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830110307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T23:41:22Z","timestamp":1699832482000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830110307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,11]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1981,11]]}},"alternative-id":["10.1002\/ajh.2830110307"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830110307","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,11]]}}}