{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:10:28Z","timestamp":1699845028633},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":8837,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1982,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study sought to delineate the phenotypic heterogeneity of the human T\u2010cell malignancies. Twenty T\u2010cell neoplasms were investigated for reactivity with the OKT hybridoma monoclonal antibodies and expression of acid \u03b1\u2010naphthyl acetate esterase (ANAE), \u03b2\u2010glucuronidase (BG), and acid phosphatase (AP) activity. Twelve cases (Mycosis fungoides, Sezary syndrome, cutaneous T\u2010cell lymphoma, chronic lymphocytic leukemia) were OKT3<jats:sup>+<\/jats:sup>T4<jats:sup>+<\/jats:sup>, ie, expressed the phenotype commonly associated with mature T\u2010helper cells. These cases were further divisible into ANAE<jats:sup>+<\/jats:sup>BG<jats:sup>+<\/jats:sup> (6 cases), ANAE<jats:sup>\u2010<\/jats:sup>BG<jats:sup>+<\/jats:sup> (5 cases), and ANAE<jats:sup>\u2010<\/jats:sup>BG<jats:sup>\u2010<\/jats:sup> (1 case) phenotypes. In contrast to the 12 OKT3<jats:sup>+<\/jats:sup>T4<jats:sup>+<\/jats:sup> cases, the remaining 8 cases showed considerable inter\u2010 and intratumor heterogeneity with respect to reactivity with the OKT antibodies. Six of these cases (acute lymphoblastic leukemia, lymphoblastic lymphoma) expressed phenotypes consistent with various intrathymic stages of T\u2010cell differentiation. Five of the latter 6 cases were AP<jats:sup>+<\/jats:sup>BG<jats:sup>+<\/jats:sup>ANAE<jats:sup>\u2010<\/jats:sup>, analogous to the majority of normal cortical thymocytes; an OKT3<jats:sup>+<\/jats:sup>T4<jats:sup>\u2010<\/jats:sup>T8<jats:sup>+<\/jats:sup>T10<jats:sup>+<\/jats:sup> neoplasm was ANAE<jats:sup>+<\/jats:sup>, analogous to normal medullary thymocytes. Two cases expressed the previously undescribed OKT3<jats:sup>+<\/jats:sup>T4<jats:sup>\u2010<\/jats:sup>T8<jats:sup>\u2010<\/jats:sup>T10<jats:sup>+<\/jats:sup> phenotype. These studies demonstrate that the T\u2010cell malignancies are divisible into phenotypes which correspond to normal maturational stages of T\u2010cell differentiation and functionally distinct T\u2010cell subsets. Phenotypic analysis of the human T\u2010cell malignancies may provide a basis for understanding their biological heterogeneity and may aid in the identification of transitional stages of T\u2010cell differentiation and minor T\u2010cell subsets.<\/jats:p>","DOI":"10.1002\/ajh.2830120305","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T07:37:43Z","timestamp":1172043463000},"page":"233-245","source":"Crossref","is-referenced-by-count":13,"title":["Phenotypic heterogeneity of human T\u2010cell malignancies: Demonstration by monoclonal antibodies and cytochemical markers"],"prefix":"10.1002","volume":"12","author":[{"suffix":"II","given":"Daniel M.","family":"Knowles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James P.","family":"Halper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genevieve 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