{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T05:10:04Z","timestamp":1695877804365},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":8349,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1983,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A monoclonal antibody of IgM class that defines the antigen present on human peripheral blood granulocytes was produced and characterized. This monoclonal antibody (IF10) was made from a single fusion between P3\u2010X63\u2010Ag8\u2010U1 (P3U1) myeloma cells and splenoctyes from a BALB\/c mouse immunized against human cultured monocytoid cell line THP\u20101 cells. IF10\u2010defined antigen(s) was expressed on the cells of granulocyte lineage such as peripheral blood granulocytes and metamyelocytes, myelocytes, promyelocytes, and a part of myeloblasts in the normal bone marrow, whereas it was not detected on resting and activated T lymphocytes, B lymphocytes, adherent monocytes, and thymocytes. The IF10\u2010defined antigen was also expressed on cultured monocytoid cell lines as well as myeloid and myeloid\/erythroid (K\u2010562) cell lines. T lymphoblastoid cell lines, particularly those representing the T cells at an early thymocyte level, and a part of null cell lines were also reactive to IF10. Therefore, IF10 may be a unique monoclonal antibody that defines an antigen(s) which is expressed on almost whole stages of granulocytes and early stages of macrophages and T\u2010cell lineages, and possibly during very early stages of erythroid lineage.<\/jats:p>","DOI":"10.1002\/ajh.2830150210","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T09:46:22Z","timestamp":1172051182000},"page":"181-194","source":"Crossref","is-referenced-by-count":1,"title":["Myeloid differentiation antigen defined by a monoclonal antibody 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