{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:31:07Z","timestamp":1762875067559},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":8045,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1984,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:sup>51<\/jats:sup>Cr\u2010labeled autologous platelets were infused into splenectomized subjects and the specific radioactivities of high\u2010density (HD) and low\u2010density (LD) platelet subpopulations were determined sequentially in postinfusion samples. A rapid decrease in the specific radioactivity of LD cohorts (T 1\/2 = 2.5 days) was observed, but the specific radioactivity of HD platelets remained constant or increased slightly during the first 4 days and then gradually declined for the next 5 days. No experimental artifacts during the platelet\u2010labeling steps that could account for these results were demonstrated. These findings confirm previous observations in eusplenic individuals and support the hypothesis that human LD platelets are, on the average, younger than HD platelets.<\/jats:p><jats:p>LD platelets contain 33.8 \u00b1 13.5 ng serotonin (5HT)\/10<jats:sup>8<\/jats:sup> platelets and HD platelets 76.8 \u00b1 9.5 ng 5HT\/10<jats:sup>8<\/jats:sup> platelets (P &lt; 0.001). Sequential measurements of 5HT in PRP platelets were performed during the recovery phase of thrombocytopenia following splenectomy in patients with idiopathic thrombocytopenic purpura (ITP), a condition associated with aging of platelets in circulation. Presplenectomy platelet 5HT was 17.7 ng\/10<jats:sup>8<\/jats:sup> platelets and on days 1, 6, and 12 after surgery it increased to 18.1, 37.8, and 61.0 ng\/10<jats:sup>8<\/jats:sup> platelets (n = 7). When three healthy volunteers were given aspirin (500 mg\/day) for up to 15 days, no significant change in the 5HT content of circulating platelets was observed. If aspirin blocks, at least partially, the secretory process in vivo without interfering the 5HT uptake by the platelets, this finding stands against the possibility that a net depletion of 5HT occurs during the life\u2010span of normal human platelets.<\/jats:p><jats:p>The observation that human HD platelets, enriched with older cells, contain more 5HT than LD platelets taken together with the parallel increase in platelet 5HT and age during the recovery from thrombocytopenia in ITP patients and the lack of effect of aspirin on platelet 5HT content, provides initial evidence that human platelets accumulate 5HT during their life\u2010span in circulation.<\/jats:p>","DOI":"10.1002\/ajh.2830170103","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:10:32Z","timestamp":1172052632000},"page":"11-21","source":"Crossref","is-referenced-by-count":22,"title":["Increase in density and accumulation of serotonin by human aging platelets"],"prefix":"10.1002","volume":"17","author":[{"given":"Diego","family":"Mezzano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Aranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaldo","family":"Foradori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Lira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"741","article-title":"Density subpopulations of rabbit platelets: Size, protein, and sialic acid content, and specific radioactivity changes following labeling with 35S\u2010sulfate in vivo","volume":"57","author":"Rand ML","year":"1981","journal-title":"Stood"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V60.1.166.166"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1182\/blood.V61.2.362.362","article-title":"Survival of density subpopulations of rabbit platelets: Use of 51Cr\u2010 or 111In\u2010labeled platelets to measure survival of least dense and most dense platelets concurrently","volume":"61","author":"Rand ML","year":"1983","journal-title":"Blood"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1182\/blood.V46.3.321.321","article-title":"Platelet size in man","volume":"46","author":"Pwilus JM","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03583.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03611.x"},{"issue":"619","key":"e_1_2_1_8_2","first-page":"197","article-title":"Cohort labelling of rat platelets with 75Se\u2010methionine","volume":"46","author":"Martin JF","year":"1981","journal-title":"Thromb Haemostas"},{"key":"e_1_2_1_9_2","first-page":"295","article-title":"The inverse relation between platelet volume and platelet number: Abnormalities in hematologic disease and evidence that platelet size does not correlate with platelet age","volume":"101","author":"Levin J","year":"1983","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(80)90033-X"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830110108"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830130307"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1982.22382224940.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104970"},{"key":"e_1_2_1_15_2","first-page":"366","article-title":"Rapid, sensitive microassay for platelet 5\u2010HT","volume":"31","author":"Drummond AH","year":"1974","journal-title":"Thromb Diathes Haemorrh"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198105073041904"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1182\/blood.V56.3.329.329","article-title":"Autoimmune thrombocytopenic purpura","volume":"56","author":"Karpatkin S","year":"1980","journal-title":"Blood"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1975.tb00855.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1182\/blood.V49.1.71.71","article-title":"Heterogeneity of human whole blood platelet subpopulations. 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