{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:38:10Z","timestamp":1760981890972},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7983,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1984,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A possible mechanism of the formation of microspherocytosis in unsplenectomized patients with hereditary spherocytosis (HS) was investigated in relation to lipid metabolism in plasma and in red cells of these patients. Plasma lipids (total cholesterol, free cholesterol, high density lipoprotein cholesterol, free fatty acids, and phospholipids) were markedly reduced in unsplenectomized HS patients with microspherocytosis. Red cell membrane lipids (free cholesterol and phospholipids such as phosphatidyl ethanolamine, sphingomyelin, phosphatidyl choline, and lysophosphatidyl choline) were also decreased in these unsplenectomized HS patients. After splenectomy, microspherocytosis disappeared concomitant to substantial normalization of plasma and red cell membrane lipids. These observations suggest that plasma lipid decrement in the unsplenectomized HS patients is at least one of the causative factors in pathogenesis of the formation of microspherocytosis. So\u2010called \u201csplenic conditioning\u201d may not imply a physical \u201closs\u201d of membrane by fragmentation or pitting of the once\u2010formed HS red cells in the peripheral circulation. Instead, the decreased plasma lipids in the presence of the spleen may affect the de novo synthesis of red cell membrane lipids, resulting in the formation of microspherocytosis.<\/jats:p>","DOI":"10.1002\/ajh.2830170205","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:09:57Z","timestamp":1172052597000},"page":"129-139","source":"Crossref","is-referenced-by-count":14,"title":["Disappearance of microspherocytes in peripheral circulation and normalization of decreased lipids in plasma and in red cells of patients with hereditary spherocytosis after splenectomy"],"prefix":"10.1002","volume":"17","author":[{"given":"Takashi","family":"Sugihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Miyashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihito","family":"Yawata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1956.00250190017001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)42152-5"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI106278"},{"key":"e_1_2_1_5_2","first-page":"457","article-title":"Scanning electron microscopy of human peripheral blood cells","volume":"35","author":"Hattori A","year":"1972","journal-title":"Acta Hematol Jpn"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-88062-9_1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/20.4.470"},{"key":"e_1_2_1_8_2","first-page":"126","article-title":"The development of an enzymatic technique for the assay of cholesterol on biological fluids","volume":"29","author":"Richmond W","year":"1972","journal-title":"Scan J Clin Invest"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-024911-4.50009-X"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(67)90269-0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)70226-3"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01747716"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1093\/ajcp\/35.3.222","article-title":"A simple method for quantitation of serum beta\u2010lipoproteins by means of the immunocrit","volume":"35","author":"Heiskell CL","year":"1961","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/S0022-2275(20)39314-7","article-title":"Improved procedure for the extraction of lipids from erythrocytes","volume":"6","author":"Rose HG","year":"1965","journal-title":"J Lipid Res"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0900374"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197203162861105"},{"key":"e_1_2_1_18_2","first-page":"816","article-title":"Hereditary plasma lecithin\u2010cholesterol acyl transferase deficiency. 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