{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:40:02Z","timestamp":1697866802912},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7922,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1984,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In ten human malignant T\u2010cell lines (HPB\u2010ALL, HPB\u2010MLT, CCRF\u2010CEM, RPMI\u20108402, MOLT\u20104, JM, P12\/Ichikawa, TALL\u20101, SKW\u20103, HUT\u2010102), the expression of the antigen defined by OKT\u20104 or Leu\u20103A monoclonal antibody was remarkably decreased after a short incubation (1 hr) with 12\u20100\u2010tetradecanoylphorbol\u201013\u2010acetate (TPA) (1.6 nM). This phenomenon was also observed in all types of cells tested, including two lymphoblastoid cell lines with B\u2010cell properties (RPMI\u20106410 and RPMI\u20108392), thumus cells from three children, peripheral blood lymphocytes from four normal individuals, and fresh neoplastic T cells from a case of T\u2010cell acute lympho\u2010blastic leukemia (ALL) and a case of T\u2010cell lymphoma. The presence of puromycin (10 \u03bcM), cycloheximide (35 \u03bcM), or retinoic acid (1.0 \u03bcM), had no effect on the TPA\u2010induced loss of OKT\u20104 defined antigen. Incubation at 4\u00b0C or with lidocaine (4 mM) partially prevented this effect of TPA, and the presence of sodium azide (150 mM) did so totally. Recovery of antigen expression occurred 24 hours after cessation of TPA treatment. Comparative study using several phorbols other than TPA showed that this effect accorded with the tumor promotor activity of the compounds, but the phenomenon appears to be at the membrane level, not necessarily as a result of TPA\u2010induced cellular differentiation.<\/jats:p>","DOI":"10.1002\/ajh.2830170309","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:07:05Z","timestamp":1172052425000},"page":"287-294","source":"Crossref","is-referenced-by-count":5,"title":["Selective loss of the expression of OKT\u20104 or leu\u20103A defined antigen by 12\u20100\u2010tetradecanoylphorbol\u201013\u2010acetate (TPA) in human lymphoid cells"],"prefix":"10.1002","volume":"17","author":[{"given":"Eiji","family":"Tatsumi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Piontek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tohru","family":"Sugimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Minato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Minowada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"153","article-title":"In vitro studies on the mode of action of the phorbol esters, potent tumor promoters. 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