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It reacted in a sensitive immunoassay of plasma fibrinogen. The specificity of the antibody was confined to the parent molecule with no significant inhibition of fibrinogen binding by the fibrinogen degradation products (FDPs) X, Y, D, or E. The antibody had no significant effect on the activated partial thromboplastin time and prothrombin time of normal plasma. However, it prolonged the thrombin time as determined by the Clauss chronometric fibrinogen method. During fibrinogen lysis by plasmin immunoreactivity of fibrinogen to the antibody was lost at the same rate as the clottable fibrinogen content determined by the Clauss assay. The lack of reactivity of the antibody with FDPs makes it a suitable reagent for investigating plasmin activity as well as studying fibrinogen and fibrin. These findings suggest that the epitope of the antibody lies within the polar protruberance of the carboxy terminal end of the A\u03b1 chain of fibrinogen and is destroyed by plasmin cleavage.<\/jats:p>","DOI":"10.1002\/ajh.2830180202","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:04:05Z","timestamp":1172052245000},"page":"111-119","source":"Crossref","is-referenced-by-count":3,"title":["Some studies with a monoclonal antibody directed against human fibrinogen"],"prefix":"10.1002","volume":"18","author":[{"given":"Susan E.","family":"Francis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas E.","family":"Joshua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Exner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry","family":"Kronenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"339","volume-title":"Fibrinogen","author":"Marder V","year":"1968"},{"key":"e_1_2_1_3_2","first-page":"903","article-title":"Immunoelectrophoretic characterization of plasma fibrinogen derivatives in patients with pathological plasma proteolysis","volume":"70","author":"Fisher S","year":"1967","journal-title":"J Lab Clin Invest"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(81)90124-9"},{"key":"e_1_2_1_5_2","first-page":"314","volume-title":"Practical Haematology","author":"Pitney R","year":"1975"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(75)90289-2"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(68)90109-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(81)90127-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1005041"},{"key":"e_1_2_1_12_2","first-page":"240","article-title":"Characterization of a crosslink\u2010containing fragment derived from the \u03b1 polymer of human fibrin and its application in immunologic studies using monoclonal antibodies","volume":"46","author":"Sobel JH","year":"1981","journal-title":"Thromb Haemost"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(83)90219-0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107183"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/258454a0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198104093041501"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1182\/blood.V53.1.81.81","article-title":"Acquired coagulation inhibitor delaying fibrinopeptide release","volume":"53","author":"Marciniak E","year":"1979","journal-title":"Blood"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830180202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830180202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:42:30Z","timestamp":1697866950000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830180202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,2]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,2]]}},"alternative-id":["10.1002\/ajh.2830180202"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830180202","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,2]]}}}