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This report describes the platelet aggregability on stimulation with botrocetin and Polybrene in four patients with platelet\u2010type von Willebrand disease (vWD) who showed increased platelet aggregation with low concentrations of ristocetin as the result of a platelet abnormality. Enhanced platelet aggregability with botrocetin was observed in platelet\u2010rich plasma (PRP) from the patients. Platelet aggregation induced by botrocetin in a mixture of normal washed platelets and patient plasma was either decreased or normal, being dependent on the amount of plasma vWF. In contrast with ristocetin and botrocetin, Polybrene did not cause increased aggregation of patient PRP. Polybrene aggregated normal washed platelets less extensively in the presence of patient plasma than normal plasma. These studies demonstrated that botrocetin induced heightened interaction between platelets and vWF, but Polybrene did not, in platelet\u2010type vWD, and that the enhanced responsiveness of patient platelets to botrocetin is related to an intrinsic platelet abnormality.<\/jats:p>","DOI":"10.1002\/ajh.2830180209","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:04:05Z","timestamp":1172052245000},"page":"179-189","source":"Crossref","is-referenced-by-count":5,"title":["Botrocetin\u2010 and polybrene\u2010induced platelet aggregation in platelet\u2010type von willebrand disease"],"prefix":"10.1002","volume":"18","author":[{"given":"Hoyu","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reizo","family":"Nagayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1653684"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107464"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109348"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.10.5317"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V55.1.9.9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108251"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1069"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI110172"},{"key":"e_1_2_1_10_2","first-page":"203","volume-title":"Progress in Hemostasis and Thrombosis","author":"Zimmerman TS","year":"1982"},{"key":"e_1_2_1_11_2","first-page":"231","volume-title":"Progress in Hemostasis and Thrombosis","author":"Hoyer LW","year":"1976"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198005083021902"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109795"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830080308"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(80)90013-4"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830100113"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198202113060603"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1182\/blood.V60.3.790.790","article-title":"Platelet\u2010type von Willebrand's disease: Characterization of a new bleeding disorder","volume":"60","author":"Miller JL","year":"1982","journal-title":"Blood"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(78)90289-X"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1182\/blood.V55.2.276.276","article-title":"Polybrene\u2010induced platelet agglutination and reduction in electrophoretic mobility: Enhancement by von Willebrand factor and inhibition by vancomycin","volume":"55","author":"Coller BS","year":"1980","journal-title":"Blood"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V61.1.99.99"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.9.4514"},{"key":"e_1_2_1_23_2","first-page":"74","article-title":"Venom coagglutinin for detection of von Willebrand factor activity in animal plasmas","volume":"101","author":"Read MS","year":"1983","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_24_2","first-page":"96","article-title":"Heterogeneity of human factor VIII, II. 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