{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:39:04Z","timestamp":1760099944843},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7465,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1986,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intravenous infusion of an analogue (f\u2010met\u2010leu\u2010phe [FMLP]) of a bacterial\u2010derived polymorphonuclear leukocyte (PMNL) chemotactic factor, or of the complementderived chemotactic stimulus, zymosan\u2010activated plasma (ZAP, containing C5a<jats:sub>des Arg<\/jats:sub>) into rabbits induces acute PMNL margination in the pulmonary vasculature. This process also occurs during hemodialysis and the adult respiratory distress syndrome. The pulmonary PMNL sequestration is accompanied by thrombocytopenia. Because of the role platelets and PMNLs play in hemostasis and defense against infection, we studied the fate of these blood elements following sequestration induced by chemotactic factors. By employing <jats:sup>111<\/jats:sup>In\u2010labelled platelets and external radioisotope scanning, platelets were found to sequester in the pulmonary vasculature during FMLP infusion. Simultaneous <jats:sup>51<\/jats:sup>Cr PMNL and <jats:sup>111<\/jats:sup> In\u2010platelet studies showed that following sequestration, PMNLs returned to the circulation and disappeared with a normal half\u2010life (T1\/2) whereas the T1\/2 of the platelets was markedly shortened (T1\/2 of control = 49 \u00b1 3.0 hr; FMLP or ZAP infused T1\/2 = 27 \u00b1 2.7 hr). Infusion of platelet\u2010activating factor (PAF) induced PMN and platelet sequestration with similar abnormalities in platelet kineties. Studies with <jats:sup>51<\/jats:sup>Cr\u2010 and <jats:sup>14<\/jats:sup>C\u2010serotonin\u2010labelled platelets showed that platelets did not release serotonin during FMLP, ZAP, or low dose PAF\u2010induced sequestration. In contrast to platelet survival, platelet size, platelet aggregation responses, and platelet glycoprotcins were not affected by transient sequestration. These results indicate that during PMNL margination induced by relatively \u201epure\u201d\ufe01 PMNL stimuli such as FMLP, platelets may reversibly marginate and subsequently be cleared at an accelerated rate. The reason for accelerated platelet clearance is not a result of circulating platelet aggregates or detectable proteolytic modification of membrane glycoproteins. Such altered platelet kinetics may contribute to thrombocytopenia during sepsis, the adult respiratory distress syndrome, and other states in which excess PMNL margination occurs.<\/jats:p>","DOI":"10.1002\/ajh.2830210205","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:16:17Z","timestamp":1172052977000},"page":"157-171","source":"Crossref","is-referenced-by-count":14,"title":["The effect of intravascular neutrophil chemotactic factors on blood neutrophil and platelet kinetics"],"prefix":"10.1002","volume":"21","author":[{"given":"Andrew C.","family":"Issekutz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murray","family":"Ripley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1982.tb21394.x"},{"key":"e_1_2_1_3_2","first-page":"8","volume-title":"Akutes Progressives Lungenversagen","author":"Staudacher C","year":"1979"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1005155"},{"key":"e_1_2_1_5_2","first-page":"454","article-title":"The lung after trauma and shock\u2010Fine structure of alveolar\u2010capillary barrier in 23 autopsies","volume":"21","author":"Pietra GG","year":"1981","journal-title":"J Trauma"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01978735"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197704072961401"},{"key":"e_1_2_1_8_2","first-page":"645","article-title":"The role of activated complement and granulocytes in shock states and myocardial infarction","volume":"98","author":"Jacob HS","year":"1981","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.118.5.1586"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1182\/blood.V51.4.731.731","article-title":"The effect of intravascular complement on granulocyte adhesiveness and distribution","volume":"51","author":"O'Flaherty JT","year":"1978","journal-title":"Blood"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108763"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1182\/blood.V61.1.125.125","article-title":"Augmentation by platelets of granulocyte aggregation in response to chemotaxins. 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