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The fibers exhibited a diameter (mean \u00b1 SD) of 17.4 \u00b1 0.62 nm and were aligned in the cell with a fiber\u2010to\u2010fiber spacing of 18.6 nm (x\u2010axis) by 22.7 nm (y\u2010 axis). Between 65 and 132 days, <jats:italic>extracellular hemoglobin crystals<\/jats:italic> developed, with a lattice periodicity of 9.63 \u00b1 0.6 nm. Fibers and crystals coexist as separate structures. These results suggest that crystal formation upon storage of packed deoxygenated sickle erythrocytes may proceed via a phase of fiber dissolution followed by hemoglobin reassembly into extracellular crystals, rather than by a progressive alignment and direct fusion of existing fibers.<\/jats:p>","DOI":"10.1002\/ajh.2830210306","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:16:12Z","timestamp":1172052972000},"page":"269-275","source":"Crossref","is-referenced-by-count":6,"title":["Fibers, crystals, and other forms of HbS polymers in deoxygenated sickle erythrocytes"],"prefix":"10.1002","volume":"21","author":[{"given":"Athanasia A.","family":"Kaperonis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean A.","family":"Handley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Chien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Corpuscles Atlas of Red Blood Cell Shapes","author":"Bessis M","year":"1974"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V58.6.1057.1057"},{"key":"e_1_2_1_4_2","first-page":"359","article-title":"Sickle cell shape and structure: Images and concepts (1840\u20131980)","volume":"8","author":"Bessis M","year":"1982","journal-title":"Blood Cells"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/272496a0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(76)80278-1"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(77)80187-3"},{"key":"e_1_2_1_8_2","series-title":"DEW Publ. 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