{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:25:27Z","timestamp":1792193127678,"version":"4.4.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7162,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1986,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A microspectrophotometric technique was used to measure the kinetics of methemoglobin reduction in intact, unaltered human erythrocytes. Reduction was catalyzed by endogenous NADPH\u2010methemoglobin reductase in the presence of Nile Blue. The technique was applied to the study of erythrocytes from a female donor with decreased glucose\u20106\u2010phosphate dehydrogenase (G\u20106\u2010PD) activity. The individual was shown to be heterozygous for deficiency of G\u20106\u2010PD. The kinetic study revealed two distinct populations of erythrocytes that were nearly equal in number. One cell population showed reduction rates between 0 and 25% of normal, whereas the second cell population displayed rates within the range seen for normal cells. Single\u2010cell indices of cell size, cell hemoglobin content, and ratio of cell hemoglobin to cell size did not correlate with single\u2010cell reduction rates and were not significantly different between the two populations. These results provide quantitative support for the X\u2010inactivation hypothesis in G\u20106\u2010PD deficiency.<\/jats:p>","DOI":"10.1002\/ajh.2830230402","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:38:51Z","timestamp":1172054331000},"page":"311-316","source":"Crossref","is-referenced-by-count":5,"title":["Kinetic analysis in single, intact cells by microspectrophotometry: Evidence for two populations of erythrocytes in an individual heterozygous for glucose\u20106\u2010phosphate dehydrogenase deficiency"],"prefix":"10.1002","volume":"23","author":[{"given":"Richard A.","family":"Ashmun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald E.","family":"Hultquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome 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Measured by Microspectrophotometry.\u201d Thesis University of Michigan 1983."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI105786"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6336(78)80001-1"}],"container-title":["American Journal of 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