{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:08:05Z","timestamp":1761894485193},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7131,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study examined the effects of platelet\u2010derived serum factors from patients with idiopathic thrombocytopenic purpura (ITP) on human umbilical cord vascular endothelial cell growth and DNA synthesis. Vascular endothelial cell growth factors were identified in the platelet extracts, and platelet\u2010derived vascular endothelial cell growth factors with molecular weights of less than 10,000 were also identified in the sera. These growth factors were stable even after 30 minutes of heat treatment at 56\u00b0C and acid treatment.<\/jats:p><jats:p>When ITP patient serum was added to vascular endothelial cells, their growth capacity and DNA synthesis capacity were lower than when serum from normal subjects or patients with ITP in remission was added. This indicated that in ITP, impairment of vascular integrity and abnormal repair of the vascular walls that accompany thrombocytopenia may be causes of the predisposition to hemorrhage. The decrease in ITP of platelet\u2010derived vascular endothelial cell growth factors with molecular weights of less than 10,000 was hypothesized to be a contributory factor.<\/jats:p>","DOI":"10.1002\/ajh.2830240103","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:38:30Z","timestamp":1172054310000},"page":"15-22","source":"Crossref","is-referenced-by-count":6,"title":["Effect of serum from patients with idiopathic thrombocytopenic purpura on cultured endothelial cell growth"],"prefix":"10.1002","volume":"24","author":[{"given":"Tokuo","family":"Nakajima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eizo","family":"Kakishita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiyoyasu","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"197","article-title":"Ultrastructural basis of experimental thrombocytopenic purpura","volume":"90","author":"Gore I","year":"1970","journal-title":"Arch Pathol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1182\/blood.V46.4.567.567","article-title":"Ultrastructural changes in endothelium associated with thrombocytopenia","volume":"46","author":"Kitchens CS","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(75)90205-4"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(77)90100-1"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(80)90020-1"},{"key":"e_1_2_1_7_2","first-page":"1551","article-title":"Role of the platelet in maintaining the integrity of the vascular endothelial cell","volume":"46","author":"Kakishita E","year":"1983","journal-title":"Acta Haematol Jpn"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V67.1.203.203"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00329383"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107470"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.23.1.101"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.4.1207"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.6.1641"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111224"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90574-1"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4481"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90107-1"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830240103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830240103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T15:00:04Z","timestamp":1697814004000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830240103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,1]]}},"alternative-id":["10.1002\/ajh.2830240103"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830240103","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1]]}}}