{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T12:10:04Z","timestamp":1697976604241},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":7100,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1987,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied the ability of B\u2010lymphocytes to differentiate to immunoglobulin\u2010producing cells in 14 patients with thalassemia major, 11 parents of the patients, and 86 normal subjects. In comparison with the parents of the patients and with the normal individuals, the patients were found to have 1) an increased number of cells spontaneously secreting immunoglobulin; 2) a decreased number of plaque\u2010forming cells (PFC) when induced with pokeweed mitogen (PWM) and a deficient helper T\u2010cell function for the induction of B\u2010cell differentiation; 3) an increase in the number of PFC after the addition of alpha interferon (100 IU\/ml) to the cell cultures with PWM. Previous incubation of non\u2010T\u2010cells with alpha interferon for 1 hr and subsequent coculture with T\u2010cells from the same patients or from normal subjects in the presence of PWM increased the number of PFC. On the contrary, previous incubation of T\u2010cells with alpha interferon followed by coculture with normal non\u2010T\u2010cells or cells from the same patient did not modify the number of PFC.<\/jats:p><jats:p>These results suggest that patients with thalassemia major possess hyperreactive spontaneous B\u2010cell responses and that these B\u2010cells are unable to differentiate to immunoglobulin\u2010secreting cells in the presence of PWM. This would be due to a T\u2010helper\u2010cell deficiency with an inability to produce lymphokines (interferon being one of them), while the B\u2010cell function seems to remain intrinsically intact.<\/jats:p>","DOI":"10.1002\/ajh.2830240206","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T10:38:25Z","timestamp":1172054305000},"page":"151-159","source":"Crossref","is-referenced-by-count":4,"title":["Effect of alpha interferon on the altered t\u2010b\u2010cell immunoregulation in patients with thalassemia major"],"prefix":"10.1002","volume":"24","author":[{"given":"Patricia","family":"Nualart","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria E.","family":"Estevez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isidro J.","family":"Ballart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana A.","family":"De Miani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Pe\u00f1alver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa","family":"Sen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000206875"},{"key":"e_1_2_1_3_2","first-page":"529","article-title":"Deficient activity of the alternative pathway of complement in beta\u2010thalassemia major","volume":"13","author":"Corry JM","year":"1981","journal-title":"Am J Dis Child"},{"key":"e_1_2_1_4_2","first-page":"317","article-title":"Phagocytosis and intracellular killing of Candida albicans by polymorphonuclear leukocytes in thalassemia","volume":"52","author":"El Falaky IH","year":"1977","journal-title":"J Egypt Public Health Assoc"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1542\/peds.60.3.349","article-title":"Defects of neutrophil chemotaxis and random migration in thalassemia major","volume":"60","author":"Kahn AJ","year":"1977","journal-title":"Pediatrics"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1182\/blood.V67.1.105.105","article-title":"Progressive dysfunction of monocytes associated with iron overload and age in patients with thalassemia major","volume":"67","author":"Ballart IJ","year":"1986","journal-title":"Blood"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-100-2-173"},{"key":"e_1_2_1_8_2","first-page":"75","article-title":"Impairment of natural killer (NK) function in carriers and patients with thalassemia major","volume":"28","author":"Goicoa MA","year":"1986","journal-title":"Nouv Rev Fr Hematol"},{"key":"e_1_2_1_9_2","first-page":"137","article-title":"Efecto de la esplenectom\u00eda sobre las poblaciones linfocitarias en talasemia mayor","volume":"30","author":"Ballart IJ","year":"1985","journal-title":"Sangre (Bare)"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.32.10.1045"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1182\/blood.V64.1.308.308","article-title":"Diminished helper\/suppressor lymphocyte ratios and natural killer activity of recipients of repeated blood transfusion","volume":"64","author":"Kaplan J","year":"1984","journal-title":"Blood"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000206598"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.253.8.1140"},{"issue":"97","key":"e_1_2_1_14_2","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood","volume":"21","author":"Boy\u00fcm A","year":"1968","journal-title":"Scand J Clin Lab Invest"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(76)90050-8"},{"key":"e_1_2_1_16_2","first-page":"196","article-title":"Clinical evaluation of B cell and T\u2010regulator cell function using a protein A haemolitic plaque assay. 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