{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:10:11Z","timestamp":1697980211887},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":6980,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1987,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study compared T\u2010cell colony formation in soft agar of lymphocytes from the peripheral blood and lymph nodes of patients with non\u2010Hodgkin's lymphoma (NHL) with T\u2010cell colony formation of peripheral blood lymphocytes from normal individuals. Mononuclear cells were separated from other blood and lymph node elements on density gradient columns, phenotyped for T\u2010 and B\u2010cell antigens using monoclonal antibodies, and then plated in soft agar cultures. Lymphocytes from peripheral blood and lymph nodes of patients with NHL exhibited less T\u2010cell colony formation (p &lt; 0.01) than did lymphocytes from normal individuals. This decrease in T\u2010cell colony formation was not the result of the number of T cells or null cells plated, or differences in proportions of T helper and T suppressor cells. However, when sera from patients with NHL were incubated with normal lymphocytes before plating in soft agar, a decrease in number of T\u2010cell colonies was observed (p &lt; 0.01). We conclude that peripheral blood and lymph node mononuclear cells from patients with NHL have a decreased ability to form T\u2010cell colonies in soft agar cultures and that this decrease is related, at least in part, to the presence of serum factor(s).<\/jats:p>","DOI":"10.1002\/ajh.2830250204","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T11:09:32Z","timestamp":1172056172000},"page":"143-153","source":"Crossref","is-referenced-by-count":1,"title":["Serum from patients with non\u2010hodgkin's lymphoma(nhl) inhibits T\u2010cell colony formation"],"prefix":"10.1002","volume":"25","author":[{"given":"Robert E.","family":"Hutchison","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane G.","family":"Tice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick 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