{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:48:47Z","timestamp":1791186527578,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":6369,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1989,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To determine if the frequently observed T celt and natural kitler dysfunction in B\u2010chronic lymphocytic leukemia might be related to the presence of large numbers of malignant B cells, we studied the effects of secretory or shed products of CLL B cells on normal (control) T cell and NK function. The cell\u2010free supernatants from CLL B cells cultured from 24 to 48 hr inhibited a variety of T cell functions including: PHA\u2010induced proliferation, PHA\u2010stimulated entry of T cells into the cell cycle, and PHA\u2010induced production of interleukin\u20102. In addition, B\u2010CLL supernatants diminished control NK activity. Purified control B cells and other malignant cell lines produced little or no inhibitory activity toward these T cell or NK functions. The sera from these same B\u2010CLL patients diminished PHA\u2010induced interleukin\u20102 production by control T cells. Initial molecular characterization of the inhibitory factor(s) revealed it to be of low molecular weight (&lt; 5000 daltons) with loss of functional activity after treatment with neuraminidase. This suggested that this substance might be either a ganglioside or glycoprotein whose inhibitory activity depends on the presence of a sialic acid moiety. If CLL B celts are capable of secreting or shedding immunosuppressive factors), then alteration of this property may result in a more normal immune system for these patients.<\/jats:p>","DOI":"10.1002\/ajh.2830300203","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T11:49:28Z","timestamp":1172058568000},"page":"61-67","source":"Crossref","is-referenced-by-count":31,"title":["Malignant chronic lymphocytic leukemia B cells elaborate soluble factors that down\u2010regulate T cell and NK function"],"prefix":"10.1002","volume":"30","author":[{"given":"Jack D.","family":"Burton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol H.","family":"Weitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil E.","family":"Kay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6427923"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1970","DOI":"10.4049\/jimmunol.130.4.1970","article-title":"IL\u20102 dependence of human NK cell activity","volume":"130","author":"Domzig WB","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910270310"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1182\/blood.V46.6.883.883","article-title":"Impaired T cell transformation in chronic lymphocytic leukemia (CLL): Demonstration of a blastogenesis inhibitory factor","volume":"46","author":"Utsinger PD","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1983.00277.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1182\/blood.V57.3.418.418","article-title":"Abnormal T\u2010cell subpopulation function in CLL: Excessive suppressor (T gamma) and deficient helper (T mu) activity with respect to B cell proliferation","volume":"57","author":"Kay NE","year":"1981","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1182\/blood.V54.2.540.540","article-title":"T\u2010cell subpopulations in chronic lymphocytic leukemia: Abnormalities in distribution and in vitro receptor maturations","volume":"54","author":"Kay NE","year":"1979","journal-title":"Blood"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"2305","DOI":"10.4049\/jimmunol.129.5.2305","article-title":"Abnormal T lymphocytes subpopulations in patients with B\u2010cell chronic lymphocytic leukemia: An analysis by monoclonal antibodies","volume":"129","author":"Platsoucas CD","year":"1982","journal-title":"J Immunol"},{"key":"e_1_2_1_10_2","first-page":"578","article-title":"Defective T cell responsiveness in chronic lymphocytic leukemia","volume":"67","author":"Kay NE","year":"1986","journal-title":"Analysis of activation events. 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