{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T10:58:33Z","timestamp":1705057113174},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":6219,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Large granular lymphocytes (LGL) may exert regulatory influences on B cell immuno\u2010globulin synthesis. We, therefore, investigated the influence of LGL from controls and B cell chronic lymphocytic leukemia patients (B\u2010CLL) on control B cell proliferation to costimulation with the F(ab\u2032)<jats:sub>2<\/jats:sub> fragment of goat antihuman \u03bc and B cell growth factor (BCGF). Purified LGL (&gt;90% by morphology) from control and B\u2010CLL peripheral blood were added in various concentrations to purified control B cells and incubated with anti\u2010\u03bc, and BCGF for 3 days. [<jats:sup>3<\/jats:sup>H]\u2010thymidine uptake of B cells was then measured. There was no proliferation of control or CLL LGL alone to the costimulatory signals of the F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of goat antihuman \u03bc chain and BCGF. Addition of control LGL to equal numbers of control B cells did not blunt control B cell responsiveness to BCGF (with control LGL 8,649\u00b1298 cpm vs. control B cells alone 8,336 \u00b1 556 cpm, mean \u00b1 SEM). When control LGL were increased to 10:1 LGL:B cell ratio, the maximal inhibition by control LGL of control B cell proliferative response to BCGF was 23%. In contrast, addition of CLL LGL at a 1:1 LGL:B cell ratio resulted in marked impairment of the control B cell proliferative response to BCGF (with CLL LGL 3,586 \u00b1 954 cpm vs. control B cells alone 8,649 \u00b1 298 cpm). Inhibition by CLL LGL occurred in a cell\u2010concentration\u2010dependent manner. No difference in CLL LGL's inhibitory effect on either resting or activated control B cell responsiveness to BCGF was noted. Inhibition of de novo protein synthesis (by cycloheximide inhibition) of CLL LGL did impair CLL LGL's inhibitory capacity for BCGF\u2010induced B cell proliferation. A possible explanation for these findings includes the possibility that a subgroup of LGL with B cell suppressive activity may have expanded as a host response to the B cell leukemia or as part of the disordered cell regulation in B\u2010CLL.<\/jats:p>","DOI":"10.1002\/ajh.2830310305","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T11:44:46Z","timestamp":1172058286000},"page":"166-172","source":"Crossref","is-referenced-by-count":5,"title":["Large granular lymphocytes from B\u2010chronic lymphocytic leukemia patients inhibit normal B cell proliferation"],"prefix":"10.1002","volume":"31","author":[{"given":"Robert T.","family":"Perri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil 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