{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:20:54Z","timestamp":1760188854184},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":5854,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1990,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thirty\u2010eight consecutive patients with a FAB\u2010classified primary myelodysplastic syndrome (MDS) were investigated for in vitro growth of colony\u2010forming units for granulocyte\u2010macrophage precursors (CFU\u2010GM) and cytogenetic analysis of bone marrow cells. Abnormal CFU\u2010GM growth was found in 30 patients (79%), and clonal chromosome abnormalities were found in 13 patients (34%). The eight patients who showed normal CFU\u2010GM growth were either cytogenetically normal (n = 5), or had a 5q\u2010 deletion (n = 3) as single or dominating karyotypic abnormality. Among the 30 patients with reduced or no colony growth, ten patients had a clonal chromosome abnormality.<\/jats:p><jats:p>Leukemia developed in eight patients. None of them grew any CFU\u2010GM colonies, and three of them were cytogenetically abnormal at the time of diagnosis of MDS.<\/jats:p><jats:p>Analysis of the bone marrow in vitro growth for CFU\u2010GM and the karyotype in patients with MDS emphasizes the close relationship between these disorders and manifest acute leukemia. Subgroups of MDS may be defined by a cytogenetic classification (e.g., the 5q\u2010 syndrome), and the CFU\u2010GM growth pattern can be of value for predicting leukemic transformation.<\/jats:p>","DOI":"10.1002\/ajh.2830340304","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T11:52:07Z","timestamp":1172058727000},"page":"175-180","source":"Crossref","is-referenced-by-count":11,"title":["Bone marrow in vitro growth and cytogenetic studies in patients with fab\u2010classified primary myelodysplastic 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