{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T07:26:18Z","timestamp":1774423578999,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":5792,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Responsiveness of bone marrow erythropoietic stem cells (CFU\u2010E and BFU\u2010E) to recombinant human erythropoietin (rh\u2010Ep) was examined in vitro in 23 patients with aplastic anemia and 14 with myelodysplastic syndrome (MDS) to investigate the clinical use of rh\u2010Ep for these diseases. Bone marrow mononuclear cells were cultured by methylcellulose methods for CFU\u2010E and BFU\u2010E assays. In normals, the CFU\u2010E numbers reached a plateau of increase at Ep doses of almost 2\u20135 units, and no further increase was observed with the addition of larger Ep doses. In aplastic anemia, the responses of CFU\u2010E to Ep were relatively good in nonsevere type and generally poor in severe type. However, the CFU\u2010E numbers increased with increasing doses of Ep in some of the patients with aplastic anemia. Among the patients with MDS, the responses of CFU\u2010E to Ep were relatively good in primary acquired refractory anemia (PARA) and primary acquired sideroblastic anemia. On the other hand, the responses of CFU\u2010E to Ep were poor in refractory anemia with an excess of blasts (RAEB) and RAEB in transformation among the MDS patients. BFU\u2010E responses to Ep were poor in severe aplastic anemia, RAEB, and RAEB\u2010T. However, there are Ep responsive patients in some of aplastic anemia and PARA. High titers of rh\u2010Ep were suggested to be effective clinically in some patients with aplastic anemia and those with PARA.<\/jats:p>","DOI":"10.1002\/ajh.2830350103","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T12:11:36Z","timestamp":1172059896000},"page":"6-12","source":"Crossref","is-referenced-by-count":23,"title":["Responsiveness of bone marrow erythropoietic stem cells (CFU\u2010E and BFU\u2010E) to recombinant human erythropoietin (rh\u2010Ep) in vitro in aplastic anemia and myelodysplastic syndrome"],"prefix":"10.1002","volume":"35","author":[{"given":"Isao","family":"Aoki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Homori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroo","family":"Chikazawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyozo","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Higashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)63387-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-116-6-2286"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198701083160207"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.9.2708"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/313806a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.22.7580"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.17.6465"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"3116","DOI":"10.1016\/S0021-9258(17)35756-3","article-title":"Structural characterization human erythropoietin","volume":"261","author":"Lai PH","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_1_10_2","first-page":"690","volume-title":"Erythropoietin: Gene cloning, protein structure, and biological properties","author":"Browne JK","year":"1986"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"17156","DOI":"10.1016\/S0021-9258(18)45504-4","article-title":"Structural characterization of natural human urinary and recombinant DNA\u2013derived erythropoietin","volume":"262","author":"Recny AM","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"12059","DOI":"10.1016\/S0021-9258(18)45317-3","article-title":"Carbohydrate structure of erythropietin expressed in chinese hamster ovary cells by a human erythropoietin cDNA","volume":"262","author":"Sasaki H","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","unstructured":"WinearlsCG OliverDO PippardMJ ReidC DowningM CotesPM:Effect of human erythropoietin derived from recombinant DNA on the anaemia of patients maintained by chronic haemodialysis. Lancet:1175 1986.","DOI":"10.1016\/S0140-6736(86)92192-6"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","unstructured":"ZinB DruekeT ZingraffJ BererhiL KreisH NaretC DelonsS CastaigneJP PeterlongoF CasadevallN VaretB:Erythropoietin treatment in anaemic patients on haemodialysis. Lancet:1329 1986.","DOI":"10.1016\/S0140-6736(86)91449-2"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198701083160203"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.295.6605.1017"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","unstructured":"MolaM MannucciPM VizzottoL CasatiS CattaneoM PonticelliC:Improvement in the haemostatic defect of uraemia after treatment with recombinant human erythropoietin. Lancet:1227 1987.","DOI":"10.1016\/S0140-6736(87)91849-6"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/039139888801100507"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1159\/000416006"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-198809000-00005"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-110-2-108"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1989.18"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V48.1.63.63"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040830218"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1968.tb15138.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.23.7574"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90682-6"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.11.3690"},{"key":"e_1_2_1_29_2","first-page":"833","article-title":"Binding of erythropoietin to CFU\u2013E derived from fetal mouse liver cells","volume":"15","author":"Fukamachi H","year":"1987","journal-title":"Exp Hematol"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1182\/blood.V69.5.1485.1485","article-title":"Binding and internalization of recombinant human erythropoietin in murine erythroid precursor cells","volume":"69","author":"Mufson RA","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113080"},{"key":"e_1_2_1_32_2","first-page":"1818","article-title":"Induction of the receptor for erythropoietin in murine erythroleukemia cells after dimethyl sulfoxide treatment","volume":"48","author":"Tojo A","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90965-3"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830110110"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.5530050304"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830350103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830350103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T15:50:18Z","timestamp":1697989818000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830350103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1002\/ajh.2830350103"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830350103","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}