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The suggested method is 100% sensitive in the diagnosis of all patients, including asymptomatic obligate carriers with hereditary spherocytosis, and is very specific in different control groups. Unlike other methods designed for the diagnosis of hereditary spherocytosis, this test does not depend on the cells' surface\u2010 area\u2010to\u2010volume ratio. Normal red blood cells that were induced to become spherocytes by different means (i.e., vinblastine, lysophosphatidyl choline, and heat) and showed increased osmotic fragility did not become susceptible to the hypertonic cryohemolysis conditions.<\/jats:p>","DOI":"10.1002\/ajh.2830350208","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T12:10:57Z","timestamp":1172059857000},"page":"104-109","source":"Crossref","is-referenced-by-count":15,"title":["Hypertonic cryohemolysis: A diagnostic test for hereditary spherocytosis"],"prefix":"10.1002","volume":"35","author":[{"given":"S.","family":"Streichman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Gesheidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Tatarsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"202","volume-title":"Practical Haematology","author":"Dacie JV","year":"1975"},{"key":"e_1_2_1_3_2","first-page":"746","article-title":"Glycerol lysis time of incubated eryth\u2013rocytes in the diagnosis of hereditary spherocytosis","volume":"84","author":"Gottfried EL","year":"1974","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb07167.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1988.tb00828.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830260111"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830310214"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830200409"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.2.471"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jss.400090315"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0308-2261(21)00462-8","article-title":"Hereditary elliptocytosis and related disorders","volume":"14","author":"Palek J","year":"1985","journal-title":"Clin Haematol"},{"key":"e_1_2_1_12_2","first-page":"2","volume-title":"Beyond Normality: The Predictive Value and Efficiency of Medical Diagnoses","author":"Galen RS","year":"1975"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(81)90038-9"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830330209"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830350208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830350208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T03:06:45Z","timestamp":1698030405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830350208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,10]]}},"alternative-id":["10.1002\/ajh.2830350208"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830350208","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,10]]}}}