{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:20:54Z","timestamp":1760188854335},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":5550,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Waldenstrom's macroglobulinemia (WM) has been hypothesized to be a pleomorphic B\u2010cell malignancy with persistent maturation towards plasma cells in all lymphoid tissue. This proposal is based on detection of a heterogeneous density of monoclonal Ig on peripheral blood B\u2010cells in patients with WM. We now present data derived from 2\u2010 and 3\u2010color immunofluorescence and flow cytometric analysis that strongly supports this hypothesis. Abnormally high numbers of B lineage cells, defined by expression of CD19, CD20, and CD24, were found among peripheral blood mononuclear cells (PBMC). These B\u2010cells are monoclonal as defined by light chain expression and by the existence of rearranged Ig genes (Southern blot analysis), although they exhibit heterogeneity in the density of surface light chain. Unlike normal PBMC B\u2010cells, the monoclonal B\u2010cells bear CD5 and CD10 (CALLA), express adhesion and adhesion\u2010related molecules (CD11b, CD9), and appear to be actively differentiating during the course of the disease, based on the pattern of CD45 isoform expression. At any given point in time, the population of monoclonal B\u2010cells is heterogeneous in differentiation stage based on transitions in the expression of CD45 isoforms from expression of CD45RA, the high molecular mass isoforms of CD45, to the low molecular mass isoform CD45R0 which appears only on very late stage B\u2010cells and early plasma cells. For one patient, analysis of CD45 isoform expression over 2 years showed that the monoclonal B\u2010cell population as a whole progressed towards terminal differentiation as defined by loss of CD45RA and acquisition of CD45R0. This indicates a continuously differentiating lineage of an unusual B\u2010cell phenotype, and\/or malignant transformation of a distinct lineage of B\u2010cells in WM.<\/jats:p>","DOI":"10.1002\/ajh.2830370106","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T12:16:26Z","timestamp":1172060186000},"page":"20-30","source":"Crossref","is-referenced-by-count":27,"title":["Transitions in CD45 isoform expression indicate continuous differentiation of a monoclonal CD5+ CD11b+ B lineage in Waldenstrom's macroglobulinemia"],"prefix":"10.1002","volume":"37","author":[{"given":"Gitte S.","family":"Jensen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Joy","family":"Andrews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Mant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rucy","family":"Vergidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey A.","family":"Ledbetter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda M.","family":"Pilarski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/j.0954-6820.1944.tb03955.x","article-title":"Incipient myelomatosis or \u201cessential\u201d hyperglobulinemia with fibrinogenopenia\u2014A new syndrome?","author":"Waldenstrom J","year":"1944","journal-title":"Acta Med Scand"},{"key":"e_1_2_1_3_2","first-page":"1985","volume-title":"Neoplastic Diseases of the Blood","author":"MacKenzie MR"},{"key":"e_1_2_1_4_2","first-page":"1127","article-title":"Immunofluoresence studies in Waldenstrom's macroglobulinemia","author":"Preu'homme JL","year":"1970","journal-title":"Rev Europ Etudes Clin et Biol"},{"key":"e_1_2_1_5_2","first-page":"710","article-title":"Immunoglobulins on the surface of lymphoid cells in Waldenstrom's macroglobulinemia","volume":"51","author":"Preu'homme J","year":"1972","journal-title":"J Clin Invest"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1980.tb00027.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1182\/blood.V61.5.911.911","article-title":"In Waldenstrom's macroglobulinemia the quantity of detectable circulating monoclonal B lymphocytes correlates with clinical course","volume":"61","author":"Smith BR","year":"1983","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830300202"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1960.00270180117016"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(87)90018-1"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"505","DOI":"10.4049\/jimmunol.136.2.505","article-title":"Leu\u20101 + (CD5 +) B\u2010cells. 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