{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:24:35Z","timestamp":1790227475951,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":5305,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recently, we described a platelet antibody against a putative collagen receptor (P62), which was found in a patient with idiopathic thrombocytopenic purpura (ITP) (Blood 69:1712). We now report a deficiency of the P62 receptor in a young man whose platelets showed defective collagen\u2010induced platelet aggregation. He had a mild bleeding tendency and slight thrombocytopenia. The results of coagulation and fibrinolysis studies were normal. The patient's platelets were partially unresponsive to collagen, although aggregation in response to ADP, thrombin, ristocetin, and calcium ionophore (A23187) was almost normal. Adhesion of his platelets to bovine collagen was markedly reduced. Addition of collagen caused no synthesis of thromboxane (TX)B<jats:sub>2<\/jats:sub>, in platelet rich plasma (PRP) from this patient. Furthermore, collagen produced no rise of cytosolic free calcium ([Ca<jats:sup>2+<\/jats:sup>]i) in fura2\u2010loaded platelets. In contrast, thrombin caused TXB<jats:sub>2<\/jats:sub>, formation and an increase of [Ca<jats:sup>2+<\/jats:sup>]i in his platelets. These results suggest defective interaction between the platelets and collagen. The lgG from the ITP\u2010patient induced irreversible aggregation in normal PRP, but caused no aggregation of the young man's platelets. Immunoblot studies showed that normal platelets had antigens with a molecular weight of 62 KDa under reducing conditions and of 57 KDa under nonreducing conditions. In contrast, the young man's platelets had no P62 band, although GPla\/lla and thrombospondin were normally present. These results indicate that impaired collagen\u2010induced aggregation in the patient's platelets was due to a deficiency of P62 and confirm that P62 may play a crucial role as a collagen receptor in platelet activation.<\/jats:p>","DOI":"10.1002\/ajh.2830390107","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T12:46:10Z","timestamp":1172061970000},"page":"25-31","source":"Crossref","is-referenced-by-count":72,"title":["Deficiency of P62, a putative collagen receptor, in platelets from a patient with defective collagen\u2010induced platelet aggregation"],"prefix":"10.1002","volume":"39","author":[{"given":"Ryukichi","family":"Ryo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinori","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Sugano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mutsumi","family":"Yasunaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuji","family":"Nakayama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyasu","family":"Saigo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Adachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Okuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"7581","DOI":"10.1016\/S0021-9258(18)34419-3","article-title":"Isolation and purification of collagen \u03b1l(l) receptor from human platelet membrane","volume":"257","author":"Chiang TM","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83918-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90073-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1182\/blood.V74.1.182.182","article-title":"Collagen\u2010Platelet interactions: Evidence for a direct interaction of collagen with platelet GPIa\/Lla and indirect interaction with platelet GPIlb\/IIIa mediated by adhesive proteins","volume":"74","author":"Coller BS","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.12.5864"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/318470a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)36098-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1182\/blood.V71.4.1074.1074","article-title":"Deficiency of intact thrombospondin and membrane glycoprotein la in platelets with defective collagen\u2010Induced aggregation and spontaneous loss of disorders","volume":"71","author":"Kehrel B","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.6.1712.1712"},{"key":"e_1_2_1_11_2","first-page":"271","article-title":"Cytosolic calcium mobilization and thromboxane synthesis in a human megakaryocytic leukemia cell","volume":"18","author":"Ryo R","year":"1990","journal-title":"Exp Hematol"},{"key":"e_1_2_1_12_2","first-page":"3805","article-title":"Elevation of intracellular calcium ion by prostaglandin El and its inhibition by protein kinase C in a human megakaryocyte leukemia cell line","volume":"49","author":"Adachi M","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(85)90041-7"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1016\/S0021-9258(18)71874-7","article-title":"Platelet plasma membrane glycoproteins. 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