{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T14:27:28Z","timestamp":1700404048249},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4880,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1993,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The DNA content of bone marrow cells in patients with acute leukemia preceded by a myelodysplastic stage (MDS\u2010AML) was compared to that in patients with de novo AML. We studied granulocytes, lymphocytes, monocytes, and blasts\/promyelocytes from Feul\u2010gen\u2010stained bone marrow smears of 11 patients with de novo AML, ten patients with MDS\u2010AML, and 13 apparently healthy controls. The mean amount of DNA per cell (DNA index; Dl) in each cell population was determined using a digital video\u2010based imageanalyzing system (CAS\u2010100). Analysis of variance (F test) showed a significant difference in the DNA content between de novo AML on one hand and MDS\u2010AML and controls on the other as regards to blasts\/promyelocytes (<jats:italic>P<\/jats:italic> &lt; 0.01), lymphocytes (<jats:italic>P<\/jats:italic> &lt; 0.05), and monocytes (<jats:italic>P<\/jats:italic> &lt; 0.01), respectively. In three of 11 (27%) patients with de novo AML, a lower than normal limit Dl was found both in immature and mature bone marrow cells. Patients with MDS\u2010AML had those of Dl values similar to normal controls. In consequence, a significantly reduced mean Dl was found in patients with de novo AML in blasts\/promyelocytes (<jats:italic>P<\/jats:italic> &lt; 0.01), and monocytes (<jats:italic>P<\/jats:italic> &lt; 0.05) compared to both normal controls and MDS\u2010AML. Together with data published separately, suggesting differences in granulocyte morphology, clonality, and HLA\u2010DR expression, these data suggest biological differences between the two diseases. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.2830420309","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:19:17Z","timestamp":1172063957000},"page":"293-298","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of acute myeloid leukemia occurring de novo or preceded by a myelodysplastic stage: Differences in cellular DNA content"],"prefix":"10.1002","volume":"42","author":[{"given":"S.","family":"Widell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Auer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Hast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Reizenstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830160203"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(87)90012-9"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(89)90142-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1992.tb00590.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.42.5.498"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198606053142302"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(87)90207-X"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1182\/blood.V60.1.172.172","article-title":"Varying involvement of peripheral granulocytes in the clonal abnormality \u2010 7 in bone marrow cells in preleukemia secondary to treatment of other malignant tumors: Cytogenetic results compared with results of flow cytometric DNA analysis and neutrophil chemotaxis","volume":"60","author":"Pedersen\u2010Bjergaard J","year":"1982","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.41.6.604"},{"key":"e_1_2_1_11_2","first-page":"40","article-title":"D\u00e9t\u00e9ction des \u00e9v\u00e9nements cellulaires rares par cytom\u00e9trie a'balayage","volume":"36","author":"Siegneurin D","year":"1988","journal-title":"Pathol Biol"},{"key":"e_1_2_1_12_2","first-page":"436","article-title":"Failure to stimulate CSA\u2010secretion in preleukemia with BCG, lithium and anabolic steroids","volume":"36","author":"Reizenstein P","year":"1984","journal-title":"J Leuk Biol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(89)90243-8"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1962.tb34656.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000206209"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1364\/AO.26.003280"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100404"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/93.3.322"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(84)90059-1"},{"key":"e_1_2_1_20_2","volume-title":"Introduction to Statistical Analysis","author":"Dixon WJ","year":"1983"},{"key":"e_1_2_1_21_2","first-page":"617","article-title":"Detection of high risk patients in chronic myelogenous leukemia by DNA\u2010image cytometry","volume":"11","author":"Muller CP","year":"1991","journal-title":"Anticancer Res"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880815)62:4<755::AID-CNCR2820620419>3.0.CO;2-3"},{"key":"e_1_2_1_23_2","unstructured":"FalleniusA:DNA\u2010content and prognosis in breast cancer. 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