{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:46:39Z","timestamp":1790419599016,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4727,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1993,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic lymphocytic leukemia (CLL) is a malignant clonal expansion of CD5+B lymphocytes. The CD5+B lymphocytes have been postulated to produce autoantibodies. CLL patients may demonstrate features of autoimmunity including autoimmune hemolytic anemia. However, the origin of the autoantibodies causing the hemolysis is not clear. The present studies were performed to determine whether these autoantibodies are the products of the neoplastic B\u2010CLL clones. Immunoglobulins (Ig) were eluted from washed red blood cells (RBC) obtained from two CLL patients at the time they had autoimmune (DAT\u2010direct antiglobulin test \u2010 positive) hemolytic anemia. The light chain phenotypes of these eluted autoantibodies were determined and found to be monotypic with exact correlation to the light chain expressed on the surface of the B\u2010CLL clones. Elutions from RBC of DAT negative patients or normal volunteers failed to demonstrate measurable amounts of Ig. In contrast, Ig eluted from RBC obtained from SLE patients with DAT positive hemolytic anemia found to be polyclonal autoantibodies exhibiting both light chain types. Furthermore, CD5+B lymphocytes obtained from the same two CLL patients (DAT+) produce, in vitro understimulation with phorbal myristate acetate (PMA), monoclonal antibodies which react and bind to RBC. Thus these studies provide direct evidence demonstrating that the antibodies causing the autoimmune hemolytic anemia in our two CLL patients are the products of the B\u2010CLL neoplastic clones.<\/jats:p>","DOI":"10.1002\/ajh.2830430406","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:13:38Z","timestamp":1172063618000},"page":"259-264","source":"Crossref","is-referenced-by-count":43,"title":["Mechanism of autoimmune hemolytic anemia in chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"43","author":[{"given":"Zev M.","family":"Sthoeger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Sthoeger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mordechai","family":"Shtalrid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Sigler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Geltner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Berrebi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197208102870603"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-113-7-525"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830130208"},{"key":"e_1_2_1_5_2","first-page":"1615","article-title":"The chronic leukemias: A review of disease manifestations and the aim of therapy","volume":"96","author":"Bergsagel DE","year":"1967","journal-title":"Can Med Assoc J"},{"key":"e_1_2_1_6_2","first-page":"216","article-title":"Clinical staging of chronic lymphocytic leukemia","volume":"46","author":"Rai KR","year":"1975","journal-title":"Blood"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.1.255"},{"key":"e_1_2_1_8_2","first-page":"49","volume-title":"Manual of Clinical Laboratory Immunology","author":"Nichols WS","year":"1986"},{"key":"e_1_2_1_9_2","first-page":"80","article-title":"Antiphospholipid antibodies in systemic lupus erythematosus: Evidence of an association with Coomb's and hypocomplementemia","volume":"15","author":"Hazeltine M","year":"1988","journal-title":"J Rheumatol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1979.tb00297.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.6.1570"},{"key":"e_1_2_1_12_2","first-page":"118","article-title":"Induction of monoclonal antibody synthesis in malignant human B cells by polyclonal B cell activators","volume":"48","author":"Robert KH","year":"1979","journal-title":"Immunol Rev"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.121.1.1"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1182\/blood.V54.2.513.513","article-title":"Monoclonal immunoglobulin rescued from a patient with chronic lymphocytic leukemia and autoimmune hemolytic anemia","volume":"54","author":"Sikora K","year":"1979","journal-title":"Blood"},{"key":"e_1_2_1_15_2","first-page":"1005","volume-title":"Hematology","author":"Silber R","year":"1990"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197709152971105"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1989.tb07643.x"},{"key":"e_1_2_1_18_2","first-page":"23s","article-title":"CD5+B lymphocytes still produce polyreactive autoantibodies after heavy chain class switching","volume":"32","author":"Wakai M","year":"1989","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V64.6.1207.1207"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.147.9.1614"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.1.202"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1986.tb01503.x"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3105056"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3105057"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2494"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.6.1539"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1182\/blood.V76.3.562.562","article-title":"Evidence that chronic lymphocytic leukemia B lymphocytes are frequently committed to production of natural autoantibodies","volume":"76","author":"Borche L","year":"1990","journal-title":"Blood"},{"key":"e_1_2_1_28_2","first-page":"809","article-title":"CD5\u2010positive B\u2010cell malignancy frequently express cross reactive idiotypes associated with IgM autoantibodies","volume":"136","author":"Kipps TJ","year":"1990","journal-title":"Am J Pathol"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1182\/blood.V72.2.422.422","article-title":"Autoantibody\u2010associated cross reactive idiotypes expressed at high frequency in chronic lymphocytic leukemia relative to B\u2010cell lymphoma of follicular center cell origin","volume":"72","author":"Kipps TJ","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.4049\/jimmunol.139.5.1727","article-title":"Isolation and characterization of human VKIII germ\u2010line genes. Implications for the molecular basis of human VKIII light chain diversity","volume":"139","author":"Chen PP","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_1_31_2","first-page":"88s","article-title":"Ig sequence analyses of IgG+ CD5+ human B cells","volume":"34","author":"Hashimoto S","year":"1991","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_32_2","first-page":"98s","article-title":"VH family usage in human autoantibodies","volume":"34","author":"Pascual V","year":"1991","journal-title":"Arthritis Rheum"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780190605"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830430406","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830430406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T04:36:53Z","timestamp":1698295013000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830430406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,8]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,8]]}},"alternative-id":["10.1002\/ajh.2830430406"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830430406","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,8]]}}}