{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:52:49Z","timestamp":1761540769803},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4515,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1994,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This report describes studies investigating the use of a collagen binding assay to improve the laboratory monitoring of desmopressin (DDAVP) therapy in patients with von Willebrand's disease (vWD). We evaluated the response of seven patients with vWD (four type I, three type IIA) to DDAVP, administered using a standard protocol, by assessing levels of von Willebrand factor (vWF) and factor VIII, as well as performing skin bleeding times (SBT) prior to, and at sequential time points following, DDAVP administration. The study employed the following assays: von Willebrand factor antigen assay (vWF:Ag; determined by ELISA); a novel functionally based collagen binding assay (CBA; determined by ELISA); ristocetin cofactor assay (RCof; determined by platelet aggregometry); von Willebrand factor muitimer analysis (using SDS\u2010agarose gels); factor VIII coagulant (FVIIIC; determined by clotting assay); and factor VIII antigen (FVIIICAG; determined by ELISA). All patients showed an initial incremental increase in vWF\/FVIII levels using all assays above, and some showed some correction in SBT. Although the absolute levels of vWF\/FVIII antigen or activity varied between patients, the CBA was found to provide consistently the greatest proportional incremental increases (i.e., \u2010fold) compared to baseline (pre\u2010 DDAVP) levels. Accordingly, we consistently observed an increase in the CBA to vWF:Ag ratio for all patients evaluated. This supplements previous findings that have suggested a unique ability of our CBA procedure to bind preferentially to higher molecular weight (i.e., more functionaily active) forms of vWF. We therefore propose that the use of the above test combination (e.g., vWF:Ag plus CBA) may provide the basis for more accurate estimation of a patient's functional responsiveness to DDAVP therapy in future studies. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.2830450303","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:48:55Z","timestamp":1172065735000},"page":"205-211","source":"Crossref","is-referenced-by-count":32,"title":["von Willebrand's disease: Use of collagen binding assay provides potential improvement to laboratory monitoring of desmopressin (DDAVP) therapy"],"prefix":"10.1002","volume":"45","author":[{"given":"Emmanuel J.","family":"Favaloro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Dean","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Grispo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Exner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry","family":"Koutts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"895","article-title":"von Willebrand factor and von Wille\u2010brand disease","volume":"70","author":"Ruggeri ZM","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_3_2","first-page":"135","volume-title":"Blood Coagulation and Haemostasis","author":"Giddings JC","year":"1985"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1182\/blood.V70.3.605.605","article-title":"Structure\u2010function relationship of human von Willebrand factor","volume":"70","author":"Girma J\u2010P","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"22777","DOI":"10.1016\/S0021-9258(18)54418-5","article-title":"Review: Von Willebrand factor","volume":"266","author":"Sadler JE","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb30985.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0268-960X(91)90032-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1182\/blood.V72.5.1449.1449","article-title":"Desmopressin: A nontransfusional form of treatment for congenital and acquired bleeding disorders","volume":"72","author":"Mannuci PM","year":"1988","journal-title":"Blood"},{"key":"e_1_2_1_9_2","first-page":"19","article-title":"Desmopressin (DDAVP) for treatment of disorders of haemostasis","volume":"8","author":"Mannucci PM","year":"1986","journal-title":"Progr Hemostas Thromb"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199104000-00011"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/00313029309084791"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(86)90150-7"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-5994.1990.tb01312.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109795"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830450303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830450303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T01:57:49Z","timestamp":1698112669000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830450303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,3]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,3]]}},"alternative-id":["10.1002\/ajh.2830450303"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830450303","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,3]]}}}