{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:26:20Z","timestamp":1759047980955},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4484,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1994,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The gene expression of myeloperoxldase (MPO), CD3\u03f5 and CD3\u03b4 molecules, the gene rearrangement of T\u2010cell receptor (TCR)\u03b4, \u03b3, and \u03b2 and Immunoglobulin heavy (IgH) chain, and the expression of cell\u2010surface antlgens were investigated In seven cases of CD7+ CD5\u2212 CD2\u2212 and four cases of CD7+ CD5+ CD2\u2212 acute lymphoblastic leukemia or lymphoblastic lymphoma (ALL\/LBL) blasts, which were negative for cytochemical myeloperoxidase (cyMPO). More mature T\u2010lineage blasts were also investigated in a comparative manner. In conclusion, the CD7+ CD5\u2212 CD2\u2212 blasts included four categories: Undifferentiated blasts without lineage commitment, T\u2010lineage blasts, T\u2010\/myeloid lineage blasts, and cyMPO\u2010negative myeioblasts. The CD7+ CD5+ CD2\u2212 blasts included two categories; T\u2010lineage and T\u2010\/myeloid lineage blasts.<\/jats:p><jats:p>The 11 cases were of the germ\u2010line gene (G) for TCR\u03b2 and IgH. Four cases were G for TCR\u03b4 and TCR\u03b3. The others were of the monoclonally rearranged gene (R) for TCR\u03b4 and G for TCR\u03b3 or R for both TCR\u03b4 and TCR\u03b3.<\/jats:p><jats:p>The expression or In vitro induction of CD13 and\/or CD33 antigens correlated with the immaturity of these neoplastic T cells, since it was observed In all 11 CD7+ CD5\u2212 CD2\u2212and CD7+ CD5+ CD2\u2212, and some CD7+ CD5+ CD2+ (CD3\u2212 CD4\u2212 COB\u2010) cases, but not in CD3\u00b1 CD4+ CD8+ or CD3+ CD4+ CD8\u2212 cases.<\/jats:p><jats:p>CD3\u03f5 mRNA, but not CD3\u03b4 mRNA, was detected in two CD7+ CD5\u2212 CD2\u2212 cases, while mRNA of neither of the two CD3 moleculea was detected In the other tested CD7+ CD5\u2212CD2\u2212 cases. In contrast, mRNA of both CD3e and CD36 were detected in ail CD7+ CD5+ CD2\u2212 cases, indicating that CD7+ CD5+ CD2\u2212 blasts at least belong to T\u2010lineage.<\/jats:p><jats:p>The blasts of two CD7+ CD5\u2212 CD2\u2212 cases with entire germ\u2010line genes and without mRNA of the three molecules (MPO, CD3\u03f5, and CD3\u03b4) were regarded as being at an undifferentiated stage prior to their commitment to either T\u2010 or myelold\u2010lineage.<\/jats:p><jats:p>The co\u2010expression of the genes of MPO and CD3e In a CD7+ CD5\u2212 CD2\u2212 case or MPO, CD3\u03f5, and CD3\u03b4 in a CD7+ CD5+ CD2\u2212 case suggested the presence of some overlapping phase for T\u2010 and myeloid\u2010lineage commitment during Immature stages of differentiation. This heips understand the conversion of some T\u2010ALL\/LBL cases to acute myeloblastic leukemia (AML). The detection of CD3\u03f5, but not CD3\u03b4 mRNA, in two CD7+ CD5\u2212 CD2\u2212cases indicated that the gene expression of CD3\u03f5 occurs at a more immature stage of differentiation than that of the CD3\u03b4 chain. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.2830450408","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:47:32Z","timestamp":1172065652000},"page":"310-320","source":"Crossref","is-referenced-by-count":15,"title":["Lineage determination of CD7+ CD5\u2212 CD2\u2212 and CD7+ CD5+ CD2\u2212 lymphoblasts: Studies on phenotype, genotype, and gene expression of myeloperoxidase, CD3\u03f5, and CD3\u03b4"],"prefix":"10.1002","volume":"45","author":[{"given":"Noriko","family":"Yoneda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Tatsumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Teshigawara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigekazu","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Nagano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Kishimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koujiro","family":"Yasunaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V60.1.1.1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V54.6.1203.1203"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113103"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V71.3.603.603"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V69.4.1062.1062"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1182\/blood.V73.5.1247.1247","article-title":"Prethymic pheno\u2010type and genotype of pre\u2010T (CD7+\/ER\u2010)\u2010cell leukemia and its chical significance within adult acute lymphoblastic leukemia","volume":"73","author":"Thiel E","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.1.253"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1182\/blood.V73.2.381.381","article-title":"CD7 +, CD4\u2010, CD8\u2010 acute leukemia: A syndrome of malignant plunpotent lymphohematopoietic cells","volume":"73","author":"Kurtzburg J","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198704303161802"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(86)90100-1"},{"key":"e_1_2_1_12_2","first-page":"21","article-title":"Frequent expression of myeloid antigen (CD13) on immature T cells in culture","volume":"53","author":"Arita Y","year":"1990","journal-title":"Acta Hematol Jpn"},{"key":"e_1_2_1_13_2","first-page":"259","volume-title":"Immunochemical analysis of the T\u2010cell specific antigens","author":"Knowles RW","year":"1986"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365516809076979"},{"key":"e_1_2_1_15_2","first-page":"1","volume-title":"Leucocyte Typing White Cell Differentiation Antigens","author":"McMichael AJ","year":"1987"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/270347a0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910210207"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1920.02620010023005"},{"key":"e_1_2_1_19_2","first-page":"102","article-title":"Ultrastructural analysis of megakaryo\u2010blasts by simultaneous detection of platelet peroxidase and platelet glycoprotein","volume":"52","author":"Kishimoto Y","year":"1989","journal-title":"Act Hematol Jpn"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_21_2","first-page":"545","volume-title":"Molecular Cloning. 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