{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T12:13:37Z","timestamp":1744287217505},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4454,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In 64 patients with primary (idiopathic) osteomyelofibrosis (OMF), a morphometric analysis has been performed on bone marrow trephine biopsies following sequential doubleimmunostaining with monoclonal antibodies against proliferating cell nuclear antigen (PCNA) and erythroid precursor cells (glycophorin C). The purpose of this study was to quantify erythropoiesis and its PCNA\u2010staining capacity and, further, to determine the impact of these parameters for the development of anemia and for prognosis. In comparison with a control group (15 patients), a significant reduction in the number of erythronormoblasts could be demonstrated, associated with an increase in PCNA\u2010labelling. Moreover, significant correlations between the amount of nucleated erythroid marrow cells and degree of anemia (hemoglobin level, hematocrit, erythrocyte count) and survival could be calculated. Adverse relationships were assessed between number of erythroid cells, thrombocyte count, and spleen size, and also argyrophilic (reticulin\/collagen) fiber density. These interactions were thought to reflect the biological behaviour of the disease process, i.e., the progression or extent of myeloid metaplasia. Our findings support ferrokinetic studies suggesting erythroid hypoplasia as one of the major causes of anemia in OMF. The remarkable high PCNA\u2010labelling index of the macrocytic\u2010megaloblastoid appearing erythropoiesis is probably caused by an overexpression of this marker protein. A comparative evaluation of Ki\u201067 antigen immunostaining in splenic tissue (myeloid metaplasia) and of the PCNA\u2010labelling in pernicious anemia lend support to the assumption of an undue prolongation of the S\u2010phase generated by secondary folate (hematinic) deficiency. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.2830460107","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:46:34Z","timestamp":1172065594000},"page":"36-42","source":"Crossref","is-referenced-by-count":9,"title":["Erythropoiesis in primary (idiopathic) osteomyelofibrosis: Quantification, PCNA\u2010reactivity, and prognostic impact"],"prefix":"10.1002","volume":"46","author":[{"given":"Juergen","family":"Thiele","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Windecker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans Michael","family":"Kvasnicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baerbel Ruth","family":"Titius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudolph","family":"Zankovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Fischer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-197109000-00001"},{"key":"e_1_2_1_3_2","first-page":"15","volume-title":"Myelofibrosis, Pathophysiology and Clinical Management","author":"Geary CG","year":"1985"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900070502"},{"key":"e_1_2_1_5_2","first-page":"228","article-title":"Idiopathic myelofibrosis: A retrospective study of 103 patients","volume":"75","author":"Anger B","year":"1990","journal-title":"Haematologica"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830340411"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1978.tb03658.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1981.tb02711.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/00313028209092127"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1983.tb02069.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-198311000-00002"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1988.tb02507.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.1990.tb00372.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428199209053572"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197710)40:4<1548::AID-CNCR2820400427>3.0.CO;2-U"},{"key":"e_1_2_1_16_2","first-page":"593","article-title":"Progressive myelofibrosis in agnogenic myeloid metaplasia","volume":"107","author":"Lohmann TP","year":"1983","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(84)80124-7"},{"key":"e_1_2_1_18_2","first-page":"51","volume-title":"Myelofibrosis, Pathophysiology and Clinical Management","author":"Frisch B","year":"1985"},{"key":"e_1_2_1_19_2","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1182\/blood.V65.4.803.803","article-title":"Myelofibrosis with myeloid metaplasia: Pathophysiologic implications of the correlation between bone marrow changes and progression of splenomegaly","volume":"65","author":"Wolf BC","year":"1985","journal-title":"Blood"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)81008-3"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428199209053584"},{"key":"e_1_2_1_22_2","first-page":"21","article-title":"Proliferating cell nuclear antigen counts in formalin\u2010fixed paraffin\u2010embedded tissue correlate with Ki\u201067 in fresh tissue","volume":"97","author":"Dervan PA","year":"1992","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_1_23_2","first-page":"6","volume-title":"Pathology of the Bone Marrow","author":"Schaefer HE","year":"1984"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/27.8.90074"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/32.2.6198355"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1988.tb02037.x"},{"key":"e_1_2_1_27_2","first-page":"676","article-title":"Methods in laboratory investigation. 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