{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:42:48Z","timestamp":1791178968535,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4240,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1994,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Plasma levels of free protein S, a vitamin K\u2010dependent anticoagulant, are decreased in persons with sickle cell anemia, but the etiology of the low levels is unknown. Protein S binds to phosphatidylserine\u2010containing phospholipids in a calcium\u2010dependent manner. Other studies have indicated that phosphatidylserine may be abnormally present on the outer surface of the membrane lipid bilayer of sickle cells and of the spectrin\u2010depleted vesicles they shed in vivo. We studied the binding of purified, radiolabeled protein S to spectrin\u2010depleted erythrocyte membrane vesicles and to density\u2010separated fractions of sickle and normal erythrocytes. Calcium\u2010dependent binding of protein S occurred with vesicles and with well\u2010aerated dense irreversibly sickled cells, but not with well\u2010aerated sickle discocytes or with normal erythrocytes. These data provide further evidence that phosphatidylserine is abnormally present on the outer surface of spectrin\u2010depleted vesicles and of irreversibly sickled cells. In addition, protein S binding to such sickle membranes in vivo may be responsible, in part, for the decreased levels of free protein S in sickle cell plasma. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajh.2830470409","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:58:10Z","timestamp":1172066290000},"page":"295-300","source":"Crossref","is-referenced-by-count":25,"title":["Erythrocyte membrane vesicles and irreversibly sickled cells bind protein S"],"prefix":"10.1002","volume":"47","author":[{"given":"Peter A.","family":"Lane","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquie L","family":"O'Connell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard 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