{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T00:03:54Z","timestamp":1698537834971},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T00:00:00Z","timestamp":1353974400000},"content-version":"vor","delay-in-days":6509,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1995,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A case of adult T\u2010cell leukemia\/lymphoma (ATL) with two different clones in the peripheral blood and lymph nodes is reported here. When cellular DNA from the lymph node was digested with <jats:italic>EcoRI<\/jats:italic>, one band larger than 9 Kb was detected. Digestion of the cellular DNA with <jats:italic>PstI<\/jats:italic> resulted in one clear band in addition to three internal fragments. In contrast, when cellular DNA from malignant peripheral blood lymphocytes (PBL) was digested with the same endonucleases, distinct bands at positions different from those observed in the lymph node were detected, indicating two separate malignant clones in the patient. Monoclonality of the tumor cells was shown by T\u2010cell receptor\u2010\u03b2 (TCR\u2010\u03b2) gene rearrangement in both PBL and lymph node. Furthermore, there was a difference in the surface phenotype between tumor cells taken from peripheral blood (CD4+, CD8\u2013) and lymph node (CD4+, CD8+). These findings suggest the presence of two different ATL clones in PBL and lymph node in a single patient simultaneously, which is distinguishable by the integration pattern of human T\u2010cell leukemia virus type I (HTLV\u2010I) proviral DNA.<\/jats:p>","DOI":"10.1002\/ajh.2830480209","type":"journal-article","created":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T23:35:22Z","timestamp":1354059322000},"page":"116-119","source":"Crossref","is-referenced-by-count":9,"title":["Adult T\u2010cell leukemia\/lymphoma with two distinct clones in the peripheral blood and lymph node"],"prefix":"10.1002","volume":"48","author":[{"given":"Keisuke","family":"Shibata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Suga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masako","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaya","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,27]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7415"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.8.2534"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3536(11)80021-1"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1182\/blood.V50.3.481.481","article-title":"Adult T\u2010cell leukemia: Clinical and hematological features of 16 cases","volume":"50","author":"Uchiyama T","year":"1977","journal-title":"Blood"},{"key":"e_1_2_1_6_2","first-page":"4644s","article-title":"Clinical diversity in adult T\u2010cell leukemia\u2010lymphoma","volume":"45","author":"Takalsuki K","year":"1985","journal-title":"Cancer Res (Suppl)"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19890115)63:2<289::AID-CNCR2820630214>3.0.CO;2-U"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428199009042466"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1991.tb08051.x"},{"key":"e_1_2_1_10_2","first-page":"273","article-title":"Defective human T\u2010cell leukemia virus in adult T\u2010cell leukemia patients","volume":"2","author":"Konishi H","year":"1984","journal-title":"Mol Biol Med"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19930801)72:3<735::AID-CNCR2820720317>3.0.CO;2-O"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1182\/blood.V84.3.853.853","article-title":"Clinical importance of extraordinary integration patterns of human T\u2010cell lymphotrophic virus type I proviral DNA in adult T\u2010cell leukemia\/lymphoma","volume":"84","author":"Shimamoto Y","year":"1994","journal-title":"Blood"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.14.4544"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410310108"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-1811(92)90062-G"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(92)90040-E"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00257100"},{"key":"e_1_2_1_18_2","first-page":"155","volume-title":"Leukaemia Cytochemistry; Principles and Practice","author":"Mirro J","year":"1989"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajh.2830480209","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.2830480209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T03:31:54Z","timestamp":1698377514000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.2830480209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,2]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,2]]}},"alternative-id":["10.1002\/ajh.2830480209"],"URL":"https:\/\/doi.org\/10.1002\/ajh.2830480209","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,2]]}}}