{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T08:10:09Z","timestamp":1698307809294},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,11]],"date-time":"2006-07-11T00:00:00Z","timestamp":1152576000000},"content-version":"vor","delay-in-days":4028,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[1995,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The immunological detection of P\u2010Glycoprotein (P\u2010GP) and the functional release of Rhodamine 123 (R123) have been compared in a number of human and murine cancer cell lines, in chemo\u2010and\/or radiotherapy\u2010resistant subclones, and in clinical specimens from patients. The chemoresistance level was established from the viability index (IC50) in the presence of doxorubicin. Cytocentrifuge preparations were immunostained with JSB\u20101 monoclonal antibody followed by the alkaline phosphatase anti\u2010alkaline phosphatase technique. The strength of the reaction was quantified by a digital image analyser. The kinetic incorporation and release of Rhodamine 123 were evaluated by flow cytometry. The parent cell lines and radiotherapy resistant subclones showed a low IC50, were JSB\u20101 negative and retained R123 during the whole experiment, while the chemoresistant and radio\u2010chemoresistant cell line mutants had a high IC50, were JSB\u20101 positive, and actively pumped the R123 out of the cells. Good correlations were obtained between the IC50, the digital image analysis, and flow cytometry. The kinetic profile of the R123 release allowed the distinction between typical and atypical multidrug resistance phenotypes. These findings were confirmed in clincal specimens from patients. We conclude that antigenic and functional studies are complementary and are useful in experimental and clinical approaches to multidrug resistance.<\/jats:p>","DOI":"10.1002\/ajh.2830490303","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T13:53:07Z","timestamp":1172065987000},"page":"183-193","source":"Crossref","is-referenced-by-count":9,"title":["Comparative study of multidrug resistance evaluated by means of the quantitative immunohistochemical detection of p\u2010glycoprotein and the functional release of rhodamine 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