{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T14:38:17Z","timestamp":1773758297096,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Acute chest syndrome (ACS) is one of the most common severe complications of sickle cell disease (SCD). In recent years, a major\u00a0role of inflammation and innate immunity has been evidenced, but ACS pathophysiology remains incompletely\u00a0understood, and therapeutic options are limited. We performed proteomic analysis of induced sputum and tracheal aspirates\u00a0in\u00a0eight\u00a0SCD children during ACS (four intubated and four non\u2010intubated) and in three during vaso\u2010occlusive crisis\u00a0(VOC) without ACS. Proteomic analysis revealed that one of the main canonical pathways involved during ACS was the complement system. To further investigate its implication, we measured the main components of the complement alternative\u00a0and\u00a0terminal pathways in sputum and plasma from SCD children during 27 ACS episodes compared with 16 VOC\u00a0episodes without ACS. A dramatic increase in the median level of C3a, C5a, sC5b\u20109, factor B, factor D, properdin, and factor H was observed in the sputum from SCD children during ACS. In the plasma, no significant increase was observed\u00a0during\u00a0ACS\u00a0compared to VOC, except for sC5b\u20109, whose median level was twofold higher than during VOC but 16\u2010fold lower\u00a0than the sC5b\u20109 median\u00a0level in the sputum during ACS. Also, the C3a\/C3 and C5a\/C5 ratios were significantly\u00a0increased in sputum compared to plasma during ACS, reflecting a predominant local pulmonary activation of the complement\u00a0system\u00a0compared to systemic activation. Our results reveal the potentially crucial role of complement activation in the lungs during ACS and open new therapeutic perspectives with anti\u2010complement agents for this severe complication of SCD.<\/jats:p>","DOI":"10.1002\/ajh.70182","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:52:01Z","timestamp":1767167521000},"page":"678-686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proteomic Analysis of Golden Sputum Reveals Pulmonary Complement Activation During Acute Chest Syndrome in Children With Sickle Cell Disease"],"prefix":"10.1002","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7068-4530","authenticated-orcid":false,"given":"Slimane","family":"Allali","sequence":"first","affiliation":[{"name":"Department of General Pediatrics and Pediatric Infectious Diseases, Sickle Cell Center, Necker\u2010Enfants Malades Hospital, Assistance 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