{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:14:52Z","timestamp":1780452892799,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n The prognostic value of\n NPM1<\/jats:italic>\n \u2010mut Measurable Residual Disease (MRD) has been increasingly recognized. However, real\u2010world data validating the prognostic impact of\n NPM1<\/jats:italic>\n \u2010mut MRD remains limited. The aim of this study was to assess the prognostic value of\n NPM1<\/jats:italic>\n \u2010mut MRD and to explore optimal MRD thresholds for relapse prediction. One hundred forty\u2010one patients with newly diagnosed\n NPM1<\/jats:italic>\n \u2010mut AML, from the Hellenic AML\u2010Registry, in CR\/CRi after the second cycle of induction and with bone marrow (BM) MRD assessment by RT\u2010qPCR, were included in our study. Allogeneic stem cell transplantation (allo\u2010SCT) in CR1 was not associated with any difference in RFS or OS between patients who achieved <\u20090.1%\n mutNPM1\/ABL<\/jats:italic>\n after the second cycle of chemotherapy, suggesting that this cut\u2010off may effectively stratify relapse risk and possibly guide optimal use of allo\u2010SCT. End\u2010of\u2010treatment (EOT) and during follow\u2010up MRD assessment exhibited equally significant prognostic value, with high\u2010level MRD positivity (\u2265\u20090.1%\n mutNPM1\/ABL<\/jats:italic>\n ) requiring early intervention to avoid imminent relapse, while those with undetectable or very low\u2010level MRD (<\u20090.01%\n mutNPM1\/ABL<\/jats:italic>\n ) being able to be safely monitored with no further intervention. Patients with low\u2010level MRD positivity (\u2265\u20090.01% and <\u20090.1%) require close MRD monitoring due to the substantial risk for progression to high\u2010level MRD positivity. Overall, our results confirm the prognostic value of MRD assessment in BM samples at certain time points, with the cut\u2010off of 0.1% after two cycles of chemotherapy enabling risk stratification and possibly informing transplant decisions, and during follow\u2010up the cut\u2010off of 0.01% being able to predict the 6\u2010month relapse risk and guide further clinical management.\n <\/jats:p>","DOI":"10.1002\/ajh.70322","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T12:35:51Z","timestamp":1775824551000},"page":"1543-1553","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Real\u2010World Outcomes in\n NPM1<\/i>\n \u2010Mutated Acute Myeloid Leukemia: The Impact of Measurable Residual Disease on Disease Relapse"],"prefix":"10.1002","volume":"101","author":[{"given":"Panagiotis","family":"Tsirigotis","sequence":"first","affiliation":[{"name":"Hematology Division, 2nd Department 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