{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:57:29Z","timestamp":1785585449218,"version":"3.56.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006396","name":"Alexion Pharmaceuticals","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The clinical significance of PNH Type II white blood cells (WBCs) remains unclear. We assessed the relative percentage (rel%) of Type II neutrophils in 355 patients with a PNH clone \u2265\u20091% on neutrophils enrolled by 33 flow cytometry laboratories in the 5\u2010year French nation\u2010wide multicenter prospective observational study. We first analyzed 127 of 133 patients with a major PNH clone (\u2265\u200950%) and evaluable Type II neutrophil rel%. Using hemolysis data available for 107 patients, a threshold of 3% Type II neutrophils rel% distinguished two groups: 22 \u201chigh Type II\u201d and 105 \u201clow Type II\u201d patients. Hemolysis was less frequent in the \u201chigh Type II\u201d group. Thrombosis was more frequent at diagnosis and during cumulative long\u2010term follow\u2010up in this group. High neutrophil rel%\u2009\u2265\u20093% was independently associated with thrombotic events. Among 222 patients with smaller PNH clones (1%\u201350%), 207 displayed evaluable Type II neutrophil rel%, of which 87 were classified as \u201chigh Type II\u201d and 120 as \u201clow Type II\u201d. Although no thrombosis was present at diagnosis, two thrombotic events occurred during follow\u2010up in the \u201chigh Type II\u201d group, whereas none were observed in the \u201clow Type II\u201d group. Long\u2010term follow\u2010up showed no major changes in Type II neutrophil rel%, irrespective of variations in total clone size. These findings suggest that, regardless of the total PNH clone size, PNH Type II neutrophil rel% is a stable parameter and may represent a reliable predictor of thrombosis, potentially supporting earlier consideration of anticomplement therapy initiation.<\/jats:p>","DOI":"10.1002\/ajh.70431","type":"journal-article","created":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T06:11:22Z","timestamp":1783145482000},"page":"2311-2328","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Proportion of\n                    <scp>PNH<\/scp>\n                    Type\n                    <scp>II<\/scp>\n                    Neutrophils Is Associated With Thrombosis in Patients Displaying a\n                    <scp>PNH<\/scp>\n                    Clone \u2265\u20091%"],"prefix":"10.1002","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9071-6683","authenticated-orcid":false,"given":"Orianne","family":"Wagner\u2010Ballon","sequence":"first","affiliation":[{"name":"D\u00e9partement H\u00e9matologie et Immunologie Biologiques APHP H\u00f4pitaux Universitaires Henri Mondor  Cr\u00e9teil France"},{"name":"Universit\u00e9 Paris\u2010Est Cr\u00e9teil (UPEC), Inserm U955 IMRB  Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Catherine","family":"Lhoumeau","sequence":"additional","affiliation":[{"name":"Institut Paoli Calmettes, Laboratoire de Biopathologie  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Raimbault","sequence":"additional","affiliation":[{"name":"APHP H\u00f4pital Saint Louis, Service H\u00e9matologie Biologique  Paris 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