{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:50:40Z","timestamp":1791337840619,"version":"4.3.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DP2HL13718601"],"award-info":[{"award-number":["DP2HL13718601"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL166249"],"award-info":[{"award-number":["R01HL166249"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015521","name":"Stanford Maternal and Child Health Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015521","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hematol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Terminal erythroid differentiation involves dramatic cellular remodeling that culminates in the expulsion of the nucleus, a process known as enucleation. While enucleation is conserved across mammals and is crucial for the generation of fully functional erythrocytes, the mechanisms governing this process have remained largely unknown, in part because the absence of genetic material in mature, enucleated red blood cells hinders genetic experimentation. Here, we performed a pooled, forward\u2010genetic CRISPR\u2010Cas9 screen in enucleated red blood cells derived from primary human hematopoietic stem\/progenitor cells to identify genes required for enucleation. We found that Chloride Intracellular Channel 3 (CLIC3) and Vesicle\u2010associated membrane protein 8 (VAMP8) are both necessary for terminal erythroid differentiation, yet likely act through different mechanisms. Knockdown of CLIC3 led to a delay in erythroblast differentiation, culminating in impaired enucleation. We found that the knockdown cells had increased p53 and p21 and exhibited cell cycle alterations, suggesting CLIC3 plays a crucial role in coordinating cell cycle progression during erythropoiesis. In comparison, VAMP8\u2010depleted cells accumulated at the orthochromatic erythroblast stage and displayed a specific defect in enucleation. Transcriptional analysis of the VAMP8\u2010knockdown cells suggested dysregulation of pathways for vesicle trafficking and actin binding, and imaging of late\u2010stage erythroblasts revealed impaired nuclear polarization and disorganized actin. This work provides a new approach for functional genomics in enucleated cells and reveals novel factors important for terminal erythroid differentiation and enucleation.<\/jats:p>","DOI":"10.1002\/ajh.70520","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:06:56Z","timestamp":1791335216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Pooled\n                    <scp>CRISPR<\/scp>\n                    Screen Reveals Genes Critical for Erythroblast Enucleation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1900-0652","authenticated-orcid":false,"given":"Marilou","family":"Tetard","sequence":"first","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5279-7507","authenticated-orcid":false,"given":"Tianjian","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana A.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah C.","family":"Gullberg","sequence":"additional","affiliation":[{"name":"Department of Biology Stanford University  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6649-8364","authenticated-orcid":false,"given":"Yann","family":"Le Guen","sequence":"additional","affiliation":[{"name":"Quantitative Services Unit, Department of Medicine Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-3748","authenticated-orcid":false,"given":"Jasmine","family":"Moshiri","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Stanford University School of Medicine  Stanford California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3707-9889","authenticated-orcid":false,"given":"John G.","family":"Doench","sequence":"additional","affiliation":[{"name":"Genetics Perturbation Platform Broad Institute of MIT and Harvard  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2112-7700","authenticated-orcid":false,"given":"Elizabeth S.","family":"Egan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford University School of Medicine  Stanford California USA"},{"name":"Department of Microbiology and Immunology Stanford University School of Medicine  Stanford California USA"},{"name":"Biohub\u2010San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"issue":"4","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"a011601","DOI":"10.1101\/cshperspect.a011601","article-title":"Erythropoiesis: Development and Differentiation","volume":"3","author":"Dzierzak E.","year":"2013","journal-title":"Cold Spring Harbor Perspectives in Medicine"},{"key":"e_1_2_12_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exphem.2023.05.001","article-title":"Dynamics of Human Hematopoietic Stem and Progenitor Cell Differentiation to the Erythroid Lineage","volume":"123","author":"Schippel N.","year":"2023","journal-title":"Experimental Hematology"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.3389\/fphys.2017.01076","article-title":"From Erythroblasts to Mature Red Blood Cells: Organelle Clearance in Mammals","volume":"8","author":"Moras M.","year":"2017","journal-title":"Frontiers in Physiology"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.exphem.2021.01.001","article-title":"Erythroid Enucleation: A Gateway Into a \u201cBloody\u201d World","volume":"95","author":"Menon V.","year":"2021","journal-title":"Experimental Hematology"},{"issue":"19","key":"e_1_2_12_6_1","doi-asserted-by":"crossref","DOI":"10.1242\/jcs.261673","article-title":"Erythroblast Enucleation at a Glance","volume":"137","author":"Newton L. M.","year":"2024","journal-title":"Journal of Cell Science"},{"issue":"9","key":"e_1_2_12_7_1","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1182\/blood-2017-05-787051","article-title":"Tropomodulin 1 Controls Erythroblast Enucleation via Regulation of F\u2010Actin in the Enucleosome","volume":"130","author":"Nowak R. B.","year":"2017","journal-title":"Blood"},{"key":"e_1_2_12_8_1","doi-asserted-by":"crossref","first-page":"100740","DOI":"10.1016\/j.blre.2020.100740","article-title":"Understanding Terminal Erythropoiesis: An Update on Chromatin Condensation, Enucleation, and Reticulocyte Maturation","volume":"46","author":"Mei Y.","year":"2021","journal-title":"Blood Reviews"},{"issue":"24","key":"e_1_2_12_9_1","doi-asserted-by":"crossref","first-page":"6258","DOI":"10.1182\/blood-2011-07-356006","article-title":"From Stem Cell to Red Cell: Regulation of Erythropoiesis at Multiple Levels by Multiple Proteins, RNAs, and Chromatin Modifications","volume":"118","author":"Hattangadi S. M.","year":"2011","journal-title":"Blood"},{"issue":"6","key":"e_1_2_12_10_1","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1002\/jha2.1051","article-title":"New Insights Into the Mechanisms of Red Blood Cell Enucleation: From Basics to Clinical Applications","volume":"5","author":"Zhuo Q.","year":"2024","journal-title":"EJHaem"},{"issue":"6235","key":"e_1_2_12_11_1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1126\/science.aaa3526","article-title":"A Forward Genetic Screen Identifies Erythrocyte CD55 as Essential for Plasmodium Falciparum Invasion","volume":"348","author":"Egan E. S.","year":"2015","journal-title":"Science"},{"issue":"11","key":"e_1_2_12_12_1","doi-asserted-by":"crossref","first-page":"2456","DOI":"10.1016\/j.cell.2023.03.035","article-title":"Massively Parallel Base Editing to Map Variant Effects in Human Hematopoiesis","volume":"186","author":"Martin\u2010Rufino J. D.","year":"2023","journal-title":"Cell"},{"issue":"1","key":"e_1_2_12_13_1","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1038\/s41467-025-58739-w","article-title":"A Genome\u2010Wide Screen Identifies Genes Required for Erythroid Differentiation","volume":"16","author":"Myers G.","year":"2025","journal-title":"Nature Communications"},{"key":"e_1_2_12_14_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.44080","article-title":"Gene\u2010Centric Functional Dissection of Human Genetic Variation Uncovers Regulators of Hematopoiesis","volume":"8","author":"Nandakumar S. K.","year":"2019","journal-title":"eLife"},{"issue":"2","key":"e_1_2_12_15_1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s12185-022-03386-w","article-title":"Induction of Enucleation in Primary and Immortalized Erythroid Cells","volume":"116","author":"Soboleva S.","year":"2022","journal-title":"International Journal of Hematology"},{"key":"e_1_2_12_16_1","doi-asserted-by":"crossref","first-page":"102221","DOI":"10.1016\/j.mib.2022.102221","article-title":"Erythrocyte\u2010Plasmodium Interactions: Genetic Manipulation of the Erythroid Lineage","volume":"70","author":"Tetard M.","year":"2022","journal-title":"Current Opinion in Microbiology"},{"issue":"19","key":"e_1_2_12_17_1","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.1182\/blood-2011-06-362038","article-title":"Proof of Principle for Transfusion of In\u00a0Vitro\u2010Generated Red Blood Cells","volume":"118","author":"Giarratana M. C.","year":"2011","journal-title":"Blood"},{"key":"e_1_2_12_18_1","doi-asserted-by":"crossref","first-page":"10","DOI":"10.7554\/eLife.61516","article-title":"Erythrocyte CD55 Mediates the Internalization of Plasmodium Falciparum Parasites","volume":"10","author":"Shakya B.","year":"2021","journal-title":"eLife"},{"issue":"2","key":"e_1_2_12_19_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1038\/nrg.2017.97","article-title":"Am I Ready for CRISPR? A User's Guide to Genetic Screens","volume":"19","author":"Doench J. G.","year":"2018","journal-title":"Nature Reviews. Genetics"},{"issue":"7","key":"e_1_2_12_20_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/s41587-020-0490-7","article-title":"Design and Analysis of CRISPR\u2010Cas Experiments","volume":"38","author":"Hanna R. E.","year":"2020","journal-title":"Nature Biotechnology"},{"issue":"23","key":"e_1_2_12_21_1","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1182\/blood.2023020831","article-title":"Plasmodium Falciparum Exploits CD44 as a Coreceptor for Erythrocyte Invasion","volume":"142","author":"Baro B.","year":"2023","journal-title":"Blood"},{"issue":"5","key":"e_1_2_12_22_1","first-page":"452","article-title":"Characterization of Murine Hemopoietic\u2010Supportive (MS\u20101 and MS\u20105) and Non\u2010Supportive (MS\u2010K) Cell Lines","volume":"6","author":"Suzuki J.","year":"1992","journal-title":"Leukemia"},{"key":"e_1_2_12_23_1","doi-asserted-by":"crossref","first-page":"101190","DOI":"10.1016\/j.xgen.2026.101190","article-title":"Balancing Off\u2010Target and On\u2010Target Considerations for Optimized CRISPR\u2010Cas9 Knockout Library Design","volume":"6","author":"Drepanos L. M.","year":"2026","journal-title":"Cell Genomics"},{"issue":"8","key":"e_1_2_12_24_1","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: A Graphical Gene\u2010Set Enrichment Tool for Animals and Plants","volume":"36","author":"Ge S. X.","year":"2020","journal-title":"Bioinformatics"},{"issue":"3","key":"e_1_2_12_25_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nmeth.4177","article-title":"Pooled CRISPR Screening With Single\u2010Cell Transcriptome Readout","volume":"14","author":"Datlinger P.","year":"2017","journal-title":"Nature Methods"},{"issue":"6","key":"e_1_2_12_26_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/s41592-020-0837-5","article-title":"Targeted Perturb\u2010Seq Enables Genome\u2010Scale Genetic Screens in Single Cells","volume":"17","author":"Schraivogel D.","year":"2020","journal-title":"Nature Methods"},{"issue":"7","key":"e_1_2_12_27_1","doi-asserted-by":"crossref","first-page":"1958","DOI":"10.1016\/j.cell.2018.10.024","article-title":"Genome\u2010Wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function","volume":"175","author":"Shifrut E.","year":"2018","journal-title":"Cell"},{"issue":"6399","key":"e_1_2_12_28_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1126\/science.aao0932","article-title":"Domain\u2010Focused CRISPR Screen Identifies HRI as a Fetal Hemoglobin Regulator in Human Erythroid Cells","volume":"361","author":"Grevet J. D.","year":"2018","journal-title":"Science"},{"issue":"5","key":"e_1_2_12_29_1","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1016\/j.celrep.2016.06.085","article-title":"Comprehensive Proteomic Analysis of Human Erythropoiesis","volume":"16","author":"Gautier E. F.","year":"2016","journal-title":"Cell Reports"},{"issue":"4","key":"e_1_2_12_30_1","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1021\/pr401191w","article-title":"Annotating N Termini for the Human Proteome Project: N Termini and Nalpha\u2010Acetylation Status Differentiate Stable Cleaved Protein Species From Degradation Remnants in the Human Erythrocyte Proteome","volume":"13","author":"Lange P. F.","year":"2014","journal-title":"Journal of Proteome Research"},{"issue":"3","key":"e_1_2_12_31_1","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1182\/blood-2005-11-007799","article-title":"In\u2010Depth Analysis of the Membrane and Cytosolic Proteome of Red Blood Cells","volume":"108","author":"Pasini E. M.","year":"2006","journal-title":"Blood"},{"issue":"6264","key":"e_1_2_12_32_1","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.aac7041","article-title":"Identification and Characterization of Essential Genes in the Human Genome","volume":"350","author":"Wang T.","year":"2015","journal-title":"Science"},{"issue":"5","key":"e_1_2_12_33_1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.exphem.2015.01.006","article-title":"Ribosomal Protein L11\u2010 and Retinol Dehydrogenase 11\u2010Induced Erythroid Proliferation Without Erythropoietin in UT\u20107\/Epo Erythroleukemic Cells","volume":"43","author":"Kummalue T.","year":"2015","journal-title":"Experimental Hematology"},{"key":"e_1_2_12_34_1","doi-asserted-by":"crossref","first-page":"14206","DOI":"10.1038\/ncomms14206","article-title":"Secreted CLIC3 Drives Cancer Progression Through Its Glutathione\u2010Dependent Oxidoreductase Activity","volume":"8","author":"Hernandez\u2010Fernaud J. R.","year":"2017","journal-title":"Nature Communications"},{"issue":"1","key":"e_1_2_12_35_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s12576-020-00740-7","article-title":"Pathophysiological Properties of CLIC3 Chloride Channel in Human Gastric Cancer Cells","volume":"70","author":"Kawai S.","year":"2020","journal-title":"Journal of Physiological Sciences"},{"issue":"1","key":"e_1_2_12_36_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41419-023-06373-z","article-title":"CLIC3 Interacts With NAT10 to Inhibit N4\u2010Acetylcytidine Modification of p21 mRNA and Promote Bladder Cancer Progression","volume":"15","author":"Shuai Y.","year":"2024","journal-title":"Cell Death & Disease"},{"issue":"1","key":"e_1_2_12_37_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.devcel.2011.11.008","article-title":"Rab25 and CLIC3 Collaborate to Promote Integrin Recycling From Late Endosomes\/Lysosomes and Drive Cancer Progression","volume":"22","author":"Dozynkiewicz M. A.","year":"2012","journal-title":"Developmental Cell"},{"issue":"1","key":"e_1_2_12_38_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1182\/blood.2019003439","article-title":"p53 Activation During Ribosome Biogenesis Regulates Normal Erythroid Differentiation","volume":"137","author":"Le Goff S.","year":"2021","journal-title":"Blood"},{"issue":"30","key":"e_1_2_12_39_1","doi-asserted-by":"crossref","first-page":"4648","DOI":"10.1038\/sj.onc.1206541","article-title":"The Onset of p53\u2010Dependent Apoptosis Plays a Role in Terminal Differentiation of Human Normoblasts","volume":"22","author":"Peller S.","year":"2003","journal-title":"Oncogene"},{"issue":"22","key":"e_1_2_12_40_1","doi-asserted-by":"crossref","first-page":"6873","DOI":"10.1182\/bloodadvances.2022007655","article-title":"Elevated CDKN1A (P21) Mediates Beta\u2010Thalassemia Erythroid Apoptosis, but Its Loss Does Not Improve Beta\u2010Thalassemic Erythropoiesis","volume":"7","author":"Liang R.","year":"2023","journal-title":"Blood Advances"},{"issue":"9","key":"e_1_2_12_41_1","doi-asserted-by":"crossref","first-page":"2567","DOI":"10.1182\/blood-2010-07-295238","article-title":"Haploinsufficiency for Ribosomal Protein Genes Causes Selective Activation of p53 in Human Erythroid Progenitor Cells","volume":"117","author":"Dutt S.","year":"2011","journal-title":"Blood"},{"issue":"8","key":"e_1_2_12_42_1","doi-asserted-by":"crossref","first-page":"R397","DOI":"10.1016\/j.cub.2018.01.005","article-title":"SNARE Complex Assembly and Disassembly","volume":"28","author":"Yoon T. Y.","year":"2018","journal-title":"Current Biology"},{"issue":"2","key":"e_1_2_12_43_1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1038\/s41580-023-00668-x","article-title":"Mechanisms of SNARE Proteins in Membrane Fusion","volume":"25","author":"Jahn R.","year":"2024","journal-title":"Nature Reviews. Molecular Cell Biology"},{"issue":"10","key":"e_1_2_12_44_1","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1038\/s41419-021-04243-0","article-title":"Prefused Lysosomes Cluster on Autophagosomes Regulated by VAMP8","volume":"12","author":"Chen Q.","year":"2021","journal-title":"Cell Death & Disease"},{"issue":"1","key":"e_1_2_12_45_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.3324\/haematol.2020.257121","article-title":"Human Erythroid Differentiation Requires VDAC1\u2010Mediated Mitochondrial Clearance","volume":"107","author":"Moras M.","year":"2022","journal-title":"Haematologica"},{"issue":"14","key":"e_1_2_12_46_1","doi-asserted-by":"crossref","first-page":"3382","DOI":"10.1182\/bloodadvances.2022008930","article-title":"EHBP1L1, an Apicobasal Polarity Regulator, Is Critical for Nuclear Polarization During Enucleation of Erythroblasts","volume":"7","author":"Wu J.","year":"2023","journal-title":"Blood Advances"},{"issue":"3","key":"e_1_2_12_47_1","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/ncb1693","article-title":"Enucleation of Cultured Mouse Fetal Erythroblasts Requires Rac GTPases and mDia2","volume":"10","author":"Ji P.","year":"2008","journal-title":"Nature Cell Biology"},{"issue":"7","key":"e_1_2_12_48_1","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.cell.2013.12.001","article-title":"Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR\/Cas System","volume":"155","author":"Chen B.","year":"2013","journal-title":"Cell"},{"key":"e_1_2_12_49_1","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1186\/s13059-015-0846-3","article-title":"Optimizing sgRNA Structure to Improve CRISPR\u2010Cas9 Knockout Efficiency","volume":"16","author":"Dang Y.","year":"2015","journal-title":"Genome Biology"},{"issue":"1","key":"e_1_2_12_50_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1002\/jbm4.10003","article-title":"Identification of Chloride Intracellular Channel Protein 3 as a Novel Gene Affecting Human Bone Formation","volume":"1","author":"Brum A. M.","year":"2017","journal-title":"JBMR Plus"},{"issue":"1","key":"e_1_2_12_51_1","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1186\/s12885-025-15205-6","article-title":"CLIC3 Emerges as a Novel Prognostic Biomarker and Therapeutic Target in Triple\u2010Negative Breast Cancer Through Integrated Multi\u2010Omics Analysis","volume":"25","author":"Yuan S.","year":"2025","journal-title":"BMC Cancer"}],"container-title":["American Journal of Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.70520","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ajh.70520","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajh.70520","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:07:02Z","timestamp":1791335222000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajh.70520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":50,"alternative-id":["10.1002\/ajh.70520"],"URL":"https:\/\/doi.org\/10.1002\/ajh.70520","archive":["Portico"],"relation":{},"ISSN":["0361-8609","1096-8652"],"issn-type":[{"value":"0361-8609","type":"print"},{"value":"1096-8652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ajh.70520"}}