{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:09:13Z","timestamp":1762542553140},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T00:00:00Z","timestamp":1465776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000857","name":"Loughborough University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Hum Biol"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Indian subpopulations (Chenchu, Koya, and Lobana Sikh) were analyzed at the genetic level for 12 <jats:italic>Alu<\/jats:italic> polymorphisms. These markers were then utilized to establish levels of genetic identity between the Indian populations and more widely between the Indian populations and a European population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Previously collected blood samples were extracted using the phenol\u2010chloroform method. The samples were utilized as templates for PCR using <jats:italic>Alu<\/jats:italic> specific primers and then analyzed by agarose gel electrophoresis for the presence and absence of the approximately 300 bp insertions. Allele frequencies were calculated by the gene counting method and were tested for Hardy\u2013Weinberg equilibrium, heterozygosities, inbreeding coefficient, and <jats:italic>G<\/jats:italic><jats:sub>ST<\/jats:sub> to assess the level of genetic differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All of the <jats:italic>Alu<\/jats:italic> loci were polymorphic in the three Indian populations studied and their average observed heterozygosity ranged from 0.294 (Lobana Sikh) to 0.357 (Koya). Allele and genotype frequency variation at the 2b, 9a, and ACE loci led to statistically significant pairwise differences among the three study populations. Overall population heterogeneity was observed for 7 out of 12 <jats:italic>Alu<\/jats:italic> polymorphisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The overall results show that these Indian samples, though displaying significant genetic variation and differences among themselves, form an Indian cluster, which as expected, is distinct from the European sample (Russian). As <jats:italic>Alus<\/jats:italic> are easily analyzed and quantified by standard and cost\u2010effective methodologies, this finding further reinforces their utility as effective population genetic markers. Am. J. Hum. Biol., 2016. \u00a9 2016 Wiley Periodicals, Inc. Am. J. Hum. Biol. 28:941\u2013944, 2016. \u00a9 2016Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ajhb.22881","type":"journal-article","created":{"date-parts":[[2016,6,13]],"date-time":"2016-06-13T09:12:56Z","timestamp":1465809176000},"page":"941-944","source":"Crossref","is-referenced-by-count":3,"title":["Genetic analysis of novel <i>Alu<\/i> insertion polymorphisms in selected indian populations"],"prefix":"10.1002","volume":"28","author":[{"given":"Susie","family":"Laybourn","sequence":"first","affiliation":[{"name":"Human Genomics Lab, School of Sport, Exercise and Heath Sciences Loughborough University, Loughborough Leicestershire LE11 3TU United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth Claire","family":"Akam","sequence":"additional","affiliation":[{"name":"Human Genomics Lab, School of Sport, Exercise and Heath Sciences Loughborough University, Loughborough Leicestershire LE11 3TU United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nick","family":"Cox","sequence":"additional","affiliation":[{"name":"Human Genomics Lab, School of Sport, Exercise and Heath Sciences Loughborough University, Loughborough Leicestershire LE11 3TU United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puneetpal","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Human Genetics Punjabi University Patiala Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarabjit S.","family":"Mastana","sequence":"additional","affiliation":[{"name":"Human Genomics Lab, School of Sport, Exercise and Heath Sciences Loughborough University, Loughborough Leicestershire LE11 3TU United 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