{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:25:11Z","timestamp":1786976711945,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"am","delay-in-days":366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["BCS\u20101638786"],"award-info":[{"award-number":["BCS\u20101638786"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011348","name":"University of Oregon","doi-asserted-by":"publisher","award":["Richard A. Bray Faculty Fellowship"],"award-info":[{"award-number":["Richard A. Bray Faculty Fellowship"]}],"id":[{"id":"10.13039\/100011348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J Hum Biol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Investigating factors that contribute to bone loss and accretion across populations in remote settings is challenging, particularly where diagnostic tools are scarce. To mitigate this challenge, we describe validation of a commercial ELISA assay to measure osteocalcin, a biomarker of bone formation, from dried blood spots (DBS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We validated the Osteocalcin Human SimpleStep ELISA kit from Abcam (ab1951214) using 158 matched plasma and DBS samples. Passing\u2010Bablok regression analysis assessed the relationships between plasma and DBS osteocalcin concentrations. Dilutional linearity and spike and recovery experiments determined if the DBS matrix interfered with osteocalcin measurement, and intra\u2010 and inter\u2010assay coefficients of variation (CVs) were calculated. Limit of detection, analyte stability, and specific forms of osteocalcin measured by the kit were also investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Mean plasma osteocalcin value was 218.2 ng\/mL (range 64.6\u2010618.1 ng\/mL). Linear relationships existed between plasma and DBS concentrations of osteocalcin, with no apparent bias in plasma vs DBS concentrations. There was no apparent interference of the DBS matrix with measurement of osteocalcin in DBS. Intra\u2010assay CV for DBS was ~8%, while average inter\u2010assay CV was 14.8%. Limit of detection was 0.34\u2009ng\/mL. Osteocalcin concentrations were stable in DBS stored at \u221228\u00b0C and room temperature, but not those stored at 37\u00b0C. This ELISA kit detects total osteocalcin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Osteocalcin, a bone formation biomarker, can be measured from DBS. Combined with a previously validated DBS assay for TRACP\u20105b, a bone resorption biomarker, these assays have the potential to help researchers disentangle the many factors contributing to bone strength.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ajhb.23394","type":"journal-article","created":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T08:42:56Z","timestamp":1580805776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Validation of an enzyme\u2010linked immunoassay assay for osteocalcin, a marker of bone formation, in dried blood spots"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7512-3265","authenticated-orcid":false,"given":"Geeta N.","family":"Eick","sequence":"first","affiliation":[{"name":"Department of Anthropology University of Oregon  Eugene Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia C.","family":"Madimenos","sequence":"additional","affiliation":[{"name":"Department of Anthropology Queens College, Flushing  New York City New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara J.","family":"Cepon\u2010Robins","sequence":"additional","affiliation":[{"name":"Department of Anthropology University of Colorado Springs  Colorado Springs Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen J.","family":"Devlin","sequence":"additional","affiliation":[{"name":"Department of Anthropology University of Michigan  Ann Arbor Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kowal","sequence":"additional","affiliation":[{"name":"Research Centre for Generational Health and Ageing University of Newcastle  Newcastle New South Wales Australia"},{"name":"Research Institute for Health Sciences Chiang Mai University  Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence S.","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Department of Anthropology University of Oregon  Eugene Oregon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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