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Here, the utility and limitations of minimally invasive sampling techniques are explored, providing a novel perspective on how reproductive hormone measurements can enhance reproductive endocrinology research. Salivary steroid measures are most commonly used, although several dried blood spot and urine assays are also available, and researchers continue to explore the efficacy of other sample types. These relatively simple measures have facilitated the collection of multiple samples from a single participant, allowing researchers to more accurately track the diurnal and cyclical variation exhibited by many reproductive hormones. Ultimately, the ability to collect fine\u2010grained participant data allows biological anthropologists to better test questions central to human reproductive ecology, life history theory, and public health. For example, fieldwork using these techniques suggests that testosterone profile variation across populations is influenced by energetic constraints and reproductive status. Moreover, hormone concentrations shape the development of sex characteristics, with implications for evolutionary questions related to sexual selection. Hormone levels also can be used to identify a range of medical concerns (eg, suppressed hormone production levels linked with psychosocial stress). These findings highlight how minimally invasive collection techniques can be applied to test diverse evolutionary hypotheses and identify important health concerns. Still, more work is needed to standardize collection and laboratory analysis procedures, thereby enabling more direct data comparisons between researchers.<\/jats:p>","DOI":"10.1002\/ajhb.23535","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T01:23:50Z","timestamp":1605057830000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Reproductive hormone measurement from minimally invasive sample types: Methodological considerations and anthropological importance"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7486-5208","authenticated-orcid":false,"given":"Theresa E.","family":"Gildner","sequence":"first","affiliation":[{"name":"Department of Anthropology Dartmouth College Hanover New Hampshire USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/01485018608986937"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jne.12590"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.2001.1786"},{"issue":"3","key":"e_1_2_11_5_1","first-page":"531","article-title":"Sex hormones, immune responses, and autoimmune diseases. 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