{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:33:48Z","timestamp":1789774428029,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J Industrial Med"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from <jats:italic>Mycobacterium immunogenum<\/jats:italic> isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of <jats:italic>M. immunogenum<\/jats:italic> and three other main species isolated from contaminated metalworking fluids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The two recombinant antigens acyl\u2010CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany. Am. J. Ind. Med. 57:195\u2013201, 2014. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ajim.22270","type":"journal-article","created":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T12:09:15Z","timestamp":1382011755000},"page":"195-201","source":"Crossref","is-referenced-by-count":9,"title":["External validation of recombinant antigens for serodiagnosis of machine operator's lung"],"prefix":"10.1002","volume":"57","author":[{"given":"Coralie","family":"Barrera","sequence":"first","affiliation":[{"name":"UMR\/CNRS 6249 Chrono\u2010Environnement University of Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Reboux","sequence":"additional","affiliation":[{"name":"UMR\/CNRS 6249 Chrono\u2010Environnement University of Franche\u2010Comt\u00e9 Besan\u00e7on France"},{"name":"Parasitology\u2010Mycology Department University Hospital of Besan\u00e7on Besan\u00e7on 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