{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T19:29:41Z","timestamp":1757705381721},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T00:00:00Z","timestamp":1117756800000},"content-version":"vor","delay-in-days":7399,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Am. J. Med. Genet."],"published-print":{"date-parts":[[1985,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The first 2,013 fetuses in 2,000 patients undergoing genetic amniocentesis at our institution were analyzed for the incidence of abnormal findings and for the safety and accuracy of the procedure. One percent of the patients were found to have aneuploid fetuses and another 1% were found to have elevated amniotic fluid concentrations of alpha\u2010fetoprotein. Advanced maternal age was the indication for amniocentesis in 84% of the women with aneuploid fetuses. Thirty\u2010two (1.6%) of the pregnancies ended in spontaneous abortion and 35 (1.7%) were terminated because of abnormal results of the prenatal diagnostic procedure. Our error rate was 0.15%, and tissue culture was successful in 97.7% of the procedures. During the latter part of our experience concurrent ultrasonography was utilized with the amniocentesis, resulting in a reduction in blood\u2010tinged specimens from 15.0% to 5.2%. In experienced hands, midtrimester amniocentesis for the purpose of prenatal diagnosis of genetically determined defects is a safe, accurate, and valuable procedure for the identification of fetal abnormalities.<\/jats:p>","DOI":"10.1002\/ajmg.1320200305","type":"journal-article","created":{"date-parts":[[2005,6,11]],"date-time":"2005-06-11T19:57:10Z","timestamp":1118519830000},"page":"443-452","source":"Crossref","is-referenced-by-count":20,"title":["Genetic amniocentesis: A twelve years' experience"],"prefix":"10.1002","volume":"20","author":[{"given":"John V.","family":"Dacus","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert S.","family":"Wilroy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Summitt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Garbaciak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas N.","family":"Abdella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Spinnato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederick W.","family":"Luthardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George S.","family":"Flinn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beverly A.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurice J.","family":"Mahoney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,3]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479-828X.1981.tb00132.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/0002-9378(79)90287-4","article-title":"Experience with sonography as an adjunct to amniocentesis for prenatal diagnosis of fetal genetic disorders","volume":"133","author":"Chandra P","year":"1979","journal-title":"Am J Obstet Gynecol"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320050405"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1979.tb10673.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320110109"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(80)90029-0"},{"key":"e_1_2_1_8_1","first-page":"5","article-title":"Amniocentesis for antenatal diagnosis of genetic defects","volume":"7","author":"Gerbie AB","year":"1980","journal-title":"Clin Obstet Gynecol"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197901253000402"},{"key":"e_1_2_1_10_1","first-page":"282","article-title":"Rates of chromosome abnormalities at different maternal ages","volume":"58","author":"Hook EB","year":"1981","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_11_1","first-page":"47","volume-title":"Perinatal Medicine: Management of the High Risk Fetus and Neonate","author":"Mennuti M","year":"1982"},{"key":"e_1_2_1_12_1","first-page":"1","article-title":"Prenatal diagnosis of neural tube defects. 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Analysis of false positive and false negative alpha\u2010fetoprotein results","volume":"48","author":"Milunsky A","year":"1976","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_14_1","first-page":"1","article-title":"Prenatal diagnosis of open neural tube defects using the amniotic fluid acetylcholinesterase activity assay","volume":"59","author":"Milunsky A","year":"1982","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(69)80023-5"},{"key":"e_1_2_1_16_1","first-page":"65","article-title":"Ultrasonography preceding diagnostic amniocentesis and its effect on amniotic fluid cell growth","volume":"50","author":"Nelson LH","year":"1977","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1001\/jama.1976.03270140023016","article-title":"Midtrimester amniocentesis for prenatal diagnosis\u2014safety and accuracy","volume":"236","author":"NICHD National Registry for Amniocentesis Study Group","year":"1976","journal-title":"JAMA"},{"key":"e_1_2_1_18_1","first-page":"739","article-title":"Prenatal diagnosis of genetic disease in Canada: Report of a collaborative study","volume":"115","author":"Simpson NE","year":"1976","journal-title":"Can Med Assoc J"},{"issue":"2","key":"e_1_2_1_19_1","first-page":"1","article-title":"An assessment of the hazards of amniocentesis","volume":"85","author":"Working Party on Amniocentesis","year":"1978","journal-title":"Br J Obstet Gynaecol"}],"container-title":["American Journal of Medical Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fajmg.1320200305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ajmg.1320200305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T15:36:49Z","timestamp":1697902609000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ajmg.1320200305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,3]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1985,3]]}},"alternative-id":["10.1002\/ajmg.1320200305"],"URL":"https:\/\/doi.org\/10.1002\/ajmg.1320200305","archive":["Portico"],"relation":{},"ISSN":["0148-7299","1096-8628"],"issn-type":[{"value":"0148-7299","type":"print"},{"value":"1096-8628","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,3]]}}}