{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T12:21:12Z","timestamp":1791030072095,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,11]],"date-time":"2007-12-11T00:00:00Z","timestamp":1197331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J of Med Genetics Pt A"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conventional tissue culturing and karyotyping of spontaneous abortions has limitations such as culture failure, external contamination and selective growth of maternal cells. Molecular cytogenetic techniques such as FISH, QF\u2010PCR, and CGH allow diagnosis on uncultured cells but are also limited as to the spectrum of cytogenetic abnormalities detected. We describe the cytogenetic findings in a series of 116 first trimester arrested pregnancies, obtained through chorionic villi sampling (CVS) and semi\u2010direct analysis that avoids some of the long\u2010culture pitfalls such as maternal contamination, and compare our results with those that would have been obtained theoretically using molecular cytogenetic techniques. Samples were obtained by transcervical CVS from women with a diagnosis of missed abortion, most of them referred for cytogenetic prenatal diagnosis. Cytogenetic analysis was performed using semi\u2010direct technique. A karyotype was obtained in 103 cases. Eighty\u2010two abnormal karyotypes were found (80%), including 12 triploidies, 10 monosomies, 61 trisomies, and 9 structural abnormalities; a double abnormality being present in 10 cases. Between 10% and 50% of our abnormal results would have been missed using the most common molecular cytogenetic techniques. Semi\u2010direct analysis of CVS may still be considered as a comprehensive, reasonably rapid, cost\u2010effective and reliable method for detecting the broadest spectrum of chromosome abnormalities in missed abortions. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ajmg.a.32058","type":"journal-article","created":{"date-parts":[[2007,12,12]],"date-time":"2007-12-12T00:19:02Z","timestamp":1197418742000},"page":"66-70","source":"Crossref","is-referenced-by-count":62,"title":["Cytogenetic study of spontaneous abortions using semi\u2010direct analysis of chorionic villi samples detects the broadest spectrum of chromosome abnormalities"],"prefix":"10.1002","volume":"146A","author":[{"given":"Carme","family":"Morales","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurora","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Bruguera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Margarit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoni","family":"Borrell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Borobio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Soler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,12,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2006.04.037"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00445-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1230"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.301"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.31552"},{"key":"e_1_2_6_7_1","first-page":"1211","article-title":"Comparative genomic hybridization in clinical cytogenetics","volume":"57","author":"Bryndorf T","year":"1995","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.4.805"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei406"},{"key":"e_1_2_6_10_1","first-page":"656","article-title":"Cytogenetic analysis of 750 spontaneous abortions with the direct\u2010preparation method of chorionic villi and its implications for studying genetic causes of pregnancy wastage","volume":"47","author":"Eiben B","year":"1990","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3310\/hta7100"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a136623"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/17.5.1166"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90615-E"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1359641"},{"key":"e_1_2_6_16_1","first-page":"53","article-title":"Early spontaneous abortion: Morphologic and karyotypic findings in 3, 912 cases","volume":"29","author":"Kalousek DK","year":"1993","journal-title":"Birth Defects Orig Artic Ser"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1295"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/302878"},{"key":"e_1_2_6_19_1","first-page":"394","article-title":"A cytogenetic study of spontaneous abortions with direct analysis of chorionic villi","volume":"77","author":"Ohno M","year":"1991","journal-title":"Obstet Gynecol"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/2524"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.826"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0223(199907)19:7<601::AID-PD564>3.0.CO;2-0"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1086\/421250"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01204052"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.115"},{"key":"e_1_2_6_26_1","first-page":"213","volume-title":"Cytogenetics of reproductive wastage: From conception to birth. 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