{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T05:41:38Z","timestamp":1761716498731},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T00:00:00Z","timestamp":1473292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 HD074695","R01 GM072859","CDC 6U01\u2010DD\u2010000489","1U01\u2010DD\u20100006982"],"award-info":[{"award-number":["R01 HD074695","R01 GM072859","CDC 6U01\u2010DD\u2010000489","1U01\u2010DD\u20100006982"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J of Med Genetics Pt A"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:label \/><jats:p>In an effort to comprehensively interrogate genetic variation in the folate pathway for risk of cleft lip with or without cleft palate (CLP), we evaluated 504 common and rare variants in 35 folate\u2010related genes in a panel of 330 infants with CLP and 367 non\u2010malformed controls. Odds ratios (OR) with 95% confidence intervals were computed for common genotypes. A Case\u2010Control Difference metric was calculated for rare variants to highlight differentially occurring alleles. Interactions between variants and a maternal folate intake variable were also evaluated. In gene\u2010only results, significant odds ratios were observed for multiple variants in the BHMT\/BHMT2\/DMGDH gene cluster, particularly in Hispanic infants. Also in this cluster, rare variant analysis highlighted a substantial case\u2010control difference in BHMT rs60340837 (synonymous Y284Y). In Hispanics, the ALDH1L1 I812V variant (rs4646750) was the most significant risk allele: OR\u2009=\u20093.8 (95%CI\u2009=\u20091.6\u20139.2) when heterozygous. In non\u2010Hispanic white infants, we observed significant risk for AHCYL2 rs1095423 (homozygous OR\u2009=\u20093.0, 95%CI 1.1\u20137.8) and the 68\u2009bp CBS insertion (c.844ins68; heterozygous OR\u2009=\u20092.4, 95%CI\u2009=\u20091.1\u20135.3). Rare variant analysis in this group revealed case\u2010control differences in MTRR and several other methionine cycle genes, a process implicated previously in clefting risk. In women with low folate intake specifically, increased risks were observed for CBS rs2851391 (OR\u2009=\u20093.6, 95%CI\u2009=\u20091.3\u20139.6) and the R259P nonsynonymous variant of TCN2 (rs1801198; OR\u2009=\u20092.8, 95%CI\u2009=\u20091.2\u20136.3). This comprehensive study provides further direction on candidate loci to help disentangle the folate\u2010related developmental phenomena in human clefting risk. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ajmg.a.37874","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T12:13:06Z","timestamp":1473336786000},"page":"2777-2787","source":"Crossref","is-referenced-by-count":16,"title":["Sequence variation in folate pathway genes and risks of human cleft lip with or without cleft palate"],"prefix":"10.1002","volume":"170","author":[{"given":"Nicholas J.","family":"Marini","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology California Institute for Quantitative Biosciences University of California Berkeley California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Stanford 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