{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T03:04:35Z","timestamp":1761102275773,"version":"3.37.3"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T00:00:00Z","timestamp":1498608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J of Med Genetics Pt A"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:label\/><jats:p>Pompe disease is a rare inherited metabolic disorder of glycogen metabolism caused by mutations in the <jats:italic>GAA<\/jats:italic> gene, encoding the acid \u03b1\u20101,4 glucosidase. Successful diagnosis of Pompe disease is achieved by clinical and biochemical evaluation followed by confirmation with DNA testing. Here, we report a male infant with a prenatal onset of cardiac symptoms and enzyme testing consistent with Pompe disease, but DNA testing by Sanger sequencing revealed no pathogenic variants. Due to the strong indication from clinical, enzymatic, and histological studies (despite the absence of molecular confirmation by traditional Sanger sequencing), enzyme replacement therapy (ERT) for Pompe disease was initiated. Reanalysis of the patient's DNA sample using next generation sequencing (NGS) of a panel of target genes causing glycogen storage disorders demonstrated compound heterozygosity for a point mutation and an exonic deletion in the <jats:italic>GAA<\/jats:italic> gene. This case illustrates the value of astute clinical judgement in patient management as well as the power of target capture deep NGS in the simultaneous detection of both a point mutation and a heterozygous exonic deletion by correcting pitfalls of the traditional PCR based sequencing, namely; allele dropout and the inability to detect exonic deletions.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ajmg.a.38333","type":"journal-article","created":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T14:32:48Z","timestamp":1498660368000},"page":"2500-2504","source":"Crossref","is-referenced-by-count":18,"title":["Next generation deep sequencing corrects diagnostic pitfalls of traditional molecular approach in a patient with prenatal onset of Pompe disease"],"prefix":"10.1002","volume":"173","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3288-3827","authenticated-orcid":false,"given":"Anne Chun\u2010Hui","family":"Tsai","sequence":"first","affiliation":[{"name":"Department of Molecular and Medical Genetics Oregon Health and Sciences University\u2010OHSU Portland Oregon"},{"name":"Section of Clinical Genetics and Metabolism, Department of Pediatrics, Children's Hospital Colorado University of Colorado Denver Aurora Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Wen","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Genetics Oregon Health and Sciences University\u2010OHSU Portland Oregon"},{"name":"Department of Pediatrics The Brooklyn Hospital Center Brooklyn New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cary","family":"Harding","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Genetics Oregon Health and Sciences University\u2010OHSU Portland Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Koeller","sequence":"additional","affiliation":[{"name":"Department of Molecular and Medical Genetics Oregon Health and Sciences University\u2010OHSU Portland Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Ambry Genetics Aliso Viejo California"},{"name":"Department of Molecular and Human Genetics Baylor College of Medicine Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee\u2010Jun C.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics Baylor College of Medicine Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5200367"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-3667"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2013.04.006"},{"key":"e_1_2_5_5_1","first-page":"1533","volume-title":"Acid maltase deficiency, myology","author":"Engel A. 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