{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T03:24:03Z","timestamp":1787282643522,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["American J of Med Genetics Pt A"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Nonimmune hydrops fetalis (NIHF) is characterized by abnormal fluid accumulation in \u2265\u20092 fetal compartments and may be genetic. The incremental diagnostic yield of prenatal exome sequencing (ES) for NIHF following a negative standard workup was previously explored on 22 cases. We report ES findings in the remaining cohort and investigate the incremental yield of genome sequencing (GS) in cases without a definitive diagnosis from exome. Enrollment criteria and exome methodology for the Hydrops\u2010Yielding Diagnostic Results of Prenatal Sequencing (HYDROPS) study were previously reported. Exomes without a definitive diagnosis were reviewed with updated clinical information and had GS if DNA was available. A genetic counselor returned the clinical reports. Overall, 50 exomes yielded 22 (44%) diagnoses and six possible diagnoses (12%). Nine new cases (9\/28, 68%) had a diagnosis involving one of the following genes:\n                      <jats:italic>RIT1, SOS1, RYR1, FLT4, LMOD2, KMT2D, PUF60,<\/jats:italic>\n                      and\n                      <jats:italic>BLTP1<\/jats:italic>\n                      . Two additional cases were diagnosed upon reclassification of uncertain variants. GS of eligible ES cases resulted in an incremental diagnostic yield of 7% (1\/14). ES and GS in NIHF significantly reduce the number of unexplained cases and guide clinical management and recurrence risks. Currently, the incremental diagnostic yield of GS over ES in NIHF remains under investigation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>ClinicalTrials.gov identifier: NCT03911531<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ajmg.a.70265","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T02:55:34Z","timestamp":1787280934000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Diagnosis Rate for Nonimmune Hydrops Fetalis With Prenatal Clinical Genome: Expanded Results From the Hydrops\u2010Yielding Diagnostic Results of Prenatal Sequencing (\n                    <scp>HYDROPS<\/scp>\n                    ) Study"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7323-7629","authenticated-orcid":false,"given":"Stephanie M.","family":"Rice","sequence":"first","affiliation":[{"name":"Division of Maternal\u2010Fetal Medicine Sidney Kimmel Medical College at Thomas Jefferson University  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7800-4894","authenticated-orcid":false,"given":"Mona M.","family":"Makhamreh","sequence":"additional","affiliation":[{"name":"Division of Maternal\u2010Fetal Medicine\/Genetics, Department of Obstetrics and Gynecology Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4060-383X","authenticated-orcid":false,"given":"Sascha","family":"Wodoslawsky","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Icahn School of Medicine at Mount Sinai  New 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