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As in other Neotropical primates, its semen characteristics and freezability are poorly studied. Hence, reproductive technologies for callitrichids would greatly benefit from reliable methods of semen analysis. In a bid to promote reproductive research in tamarins, we validated simple and inexpensive sperm function tests that can be used to monitor sperm\u2010egg binding, plasma membrane and acrosome integrity, mitochondrial activity, and DNA fragmentation. Ejaculates from adult males were individually diluted and divided into control and damage\u2010induced aliquots, and then samples comprising assorted amounts of damaged spermatozoa were examined by organelle\u2010specific tests. Our findings showed that sperm\u2010binding in chicken egg perivitelline membrane (EPM) positively correlated with the number of spermatozoa injured by snap\u2010freezing. Eosin\u2010nigrosin (EN) and propidium iodide readings were correlated with each other, and both provided robust measurements of plasma membrane integrity. A high correlation between expected and measured amounts of acrosome\u2010intact spermatozoa was found using Fast Green\u2010Rose Bengal (FG\u2010RB), Coomassie Blue (CB), and FITC\u2010PSA stains, and all three methods exhibited comparable results. Likewise, different percentages of UV\u2010irradiated spermatozoa were accurately assessed for DNA integrity by Toluidine Blue (TB) and sperm chromatin dispersion (SCD) tests. Comparisons between 3,3\u2032\u2010diaminobenzidine (DAB) and JC\u20101 stains also indicated the reliability of the former assay to ascertain gradual increases in spermatozoa with greater mitochondrial function. These data confirmed that different parts of the tamarin spermatozoa can be simply and consistently evaluated by EPM, EN, FG\u2010RB, CB, TB, and DAB protocols.<\/jats:p>","DOI":"10.1002\/ajp.23034","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T11:29:46Z","timestamp":1563449386000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of different sperm functional tests in golden\u2010headed lion tamarins (<i>Leontopithecus chrysomelas<\/i>)"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7153-2421","authenticated-orcid":false,"given":"Paloma Rocha","family":"Arakaki","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction, College of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Andrea Borges","family":"Salgado","sequence":"additional","affiliation":[{"name":"Assisted Reproduction Unit, Applied Research Department S\u00e3o Paulo Zoological Park Foundation  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Diego","family":"de Agostini Losano","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, College of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel Henrique","family":"Blank","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, College of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc\u00edlio","family":"Nichi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, College of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Jos\u00e9 Garcia","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction, College of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19991101)284:6<696::AID-JEZ11>3.0.CO;2-E"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2005.00630.x"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000480501"},{"key":"e_1_2_7_1_5_1","unstructured":"Arakaki P. 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